中国组织工程研究Issue(7)：1238-1242,5.DOI:10.3969/j.issn.2095-4344.2013.07.018
人KLF4基因克隆及重组KLF4慢病毒表达载体的构建*☆
Clone of human KLF4 gene and construction of recombinant KLF4 lentiviral vector
摘要
Abstract
BACKGROUND: KLF4 is an essential gene for cel differentiation. OBJECTIVE: To construct a lentiviral vector pCDH-KLF4 gene. METHODS: KLF4 gene amplification was used by PCR. Gene amplification products were inserted into the lentiviral vector pCDH and to co-transfect 293TN cells. DNA sequencing was used to confirm the recombinant vector. The virus supernatant was harvested and titrated. The expression of KLF4 was detected by reverse transcription-PCR. RESULTS AND CONCLUSION: DNA sequencing confirmed that the sequence of amplified KLF4 gene was consistent with the Genebank data. In transfected 5637 cells, KLF4 mRNA was over-expressed. Results verified that lentiviral vevtors of KLF4 gene over-expression were successful y constructed.关键词
组织构建/组织构建细胞学实验/慢病毒载体/KLF4 基因/基因重组/5637 细胞/载体质粒/DNA测序/组织工程/泌尿系肿瘤/大肠杆菌/DH5a 感受态细胞/国家自然科学基金/组织构建图片文章分类
医药卫生引用本文复制引用
王博涵,余虓,余淦,李恒,肖巍,徐华,叶章群..人KLF4基因克隆及重组KLF4慢病毒表达载体的构建*☆[J].中国组织工程研究,2013,(7):1238-1242,5.基金项目
国家自然科学基金-青年基金资助项目(81000292)。Supported by：the National Natural Science Foundation of China (Youth Fund), No.81000292* （81000292）
