中国组织工程研究Issue(11)：1951-1958,8.DOI:10.3969/j.issn.2095-4344.2013.11.008
低氧训练与低氧大鼠心肌细胞凋亡及凋亡因子的表达***☆
Hypoxia training regulates myocardial apoptosis and apoptotic factor expression in rats
摘要
Abstract
BACKGROUND: During hypoxia training, the body should bear the motor load and stay in the hypoxic environment outside. OBJECTIVE: To observe the effect of hypoxia and hypoxia training on myocardial apoptosis and the expression of Bax and Bcl-2 in rats. METHODS: Sixty Sprague-Dawley rats were randomly divided into six groups: normoxia group, 8 hours hypoxia group, 12 hours hypoxia group, normoxia training group, 8 hours hypoxia training group and 12 hours hypoxia training group, 10 rats in each group. The rats of normoxia training group, 8 hours hypoxia training group and 12 hours hypoxia training group were introduced to treadmil running on an incline of 0° at 25 m/min for 1 hour. After training, the rats of group 8 hours hypoxia group, 8 hours hypoxia training group, 12 hours hypoxia group and 12 hours hypoxia training group were exposed to hypoxic chamber with 12.5% volume fraction of oxygen (equal to altitude 4000 m) for 8 hours and 12 hours every day respectively. The course of the experiment was 4 weeks, 5 days every week. At 24 hours after the final experiment, the rats were treated with intraperitoneal anesthesia of sumianxin Ⅱ to obtain the samples, then hematoxylin-eosin staining, in situ terminal deoxyribonucleotide transferase-mediated dUTP nick end labeling method and protein immunohistochemistry methods were used to detect the myocardial apoptosis and the expression of Bax and Bcl-2 in rats. RESULTS AND CONCLUSION: Compared with the normoxia group, the myocardial apoptosis index was significantly increased in the 24 hours hypoxia group, normoxia training group and hypoxia training group (P <0.05); the myocardial apoptosis index in the 12 hours hypoxia training group was significantly higher than that in the normoxia training group and 8 hours hypoxia training group (P < 0.05). Compared with normoxia group, the expressions of Bcl-2, Bax and Bcl-2/Bax in the other five groups were significantly increased (P < 0.05); the expressions of Bcl-2, Bax and Bcl-2/Bax in normoxia training group were significantly higher than those in the 8 hours hypoxia group, and significantly lower than those in the 12 hours hypoxia group (P < 0.05); the expressions of Bcl-2, Bax and Bcl-2/Bax in the 12 hours hypoxia training group were significantly higher than those in the 12 hours hypoxia group and the 8 hours hypoxia training group (P < 0.05). Hypoxia and hypoxia training could induce the protein expressions of Bcl-2 and Bax in myocardial tissue. Hypoxia training was related with apoptosis rate and apoptotic index and pathological damage, especial y the 12 hours hypoxia training. Bcl-2 and Bax participated in regulating the myocardial apoptosis.关键词
组织构建/组织构建与生物活性因子/细胞凋亡/低氧/低氧运动/缺氧诱导因子 1a/Bcl-2/Bax/心肌细胞/省级基金/组织构建图片文章Key words
tissue construction/tissue construction and bioactive factors/apoptosis/hypoxia/hypoxia training/hypoxia-inducible factor 1a/Bcl-2/Bax/myocardial cells/provincial grants-supported paper/tissue construction photographs-containing paper分类
医药卫生引用本文复制引用
林喜秀,周桔,罗自强,瞿树林,赵用强,邱继旺..低氧训练与低氧大鼠心肌细胞凋亡及凋亡因子的表达***☆[J].中国组织工程研究,2013,(11):1951-1958,8.基金项目
湖南省科技厅计划项目资助(2012FJ4099)；中南大学博士后启动基金资助项目(2012，5)；2012年湖南省博士后日常经费资助(2012，11)。Supported by：Planning Project of Science and Technology Department of Hunan Province, No.2012FJ4099* （2012FJ4099）
Postdoctoral Starting Foundation of Central South University, No.2012,5* （）
Postdoctoral Daily Foundation of Hunan Province in 2012, No.2012,11* （）
