中国组织工程研究Issue(11)：2026-2031,6.DOI:10.3969/j.issn.2095-4344.2013.11.019
病毒硫氧还蛋白基因在Neuro2A细胞中的重组与表达★
Thioredoxin gene recombination and expression in Neuro-2A cells
汪龙 1吕国枫1
作者信息
- 1. 大连医科大学运动医学教研室，辽宁省大连市116000
- 折叠
摘要
Abstract
BACKGROUND: Studies on antioxidant genes Thioredoxin (TRX) have attracted more attention gradual y, but relevant studies from the aspect of gene therapy are fewer. OBJECTIVE: To study the protective effect of cells expressing the corresponding proteins in TRX transfected Neuro-2A cells and to analyze the possible mechanism underlying the protective effect. METHODS: Plasmids PIRES2-EGFP-TRX were used to transfect Neuro-2A cells and to establish a cel line which could stably express TRX proteins identified by reverse transcription-PCR. Different concentrations of hydrogen peroxide were used to treat normal cells and transfected cells for the establishment of oxidative stress models. Cel morphology, cellsurvival, glutathione concentration and DNA strand breaks situation were observed after oxidative damage. RESULTS AND CONCLUSION: Normal cells and transfected cells were both damaged by hydrogen peroxide; however, the transfected cells were superior to the normal cells in cel damage, cellsurvival, intracel ular glutathione level and degree of DNA strand breaks. These findings indicate that TRX gene in Neuro-2A cells can be reconstructed and expressed successful y, which plays a certain protective. This effect may be achieved through scavenging oxygen free radicals, maintaining intracel ular glutathione levels, thereby protecting cel ular DNA against oxidative damage.关键词
组织构建/组织构建细胞学实验/硫氧还蛋白基因/转染/Neuro-2A 细胞/氧化性应激/保护/氧自由基/还原型谷胱甘肽/应激模型/单细胞凝胶电泳/组织构建图片文章分类
医药卫生引用本文复制引用
汪龙,吕国枫..病毒硫氧还蛋白基因在Neuro2A细胞中的重组与表达★[J].中国组织工程研究,2013,(11):2026-2031,6.
