中国组织工程研究Issue(11)：2048-2053,6.DOI:10.3969/j.issn.2095-4344.2013.11.022
川芎嗪干预钝性肺挫伤急性期大鼠肺组织细胞的凋亡*
Ligustrazine prevents lung cell apoptosis in the acute stage of pulmonary contusion induced by blunt chest trauma
摘要
Abstract
BACKGROUND: Pulmonary contusion induced by blunt chest trauma can result in respiratory dysfunction and secondary inflammatory reaction, which can take part in the occurrence of systemic inflammatory response syndrome and multiple-organ dysfunction syndrome. However, the reason and mechanism of pulmonary contusion is urgently for clarity. OBJECTIVE: To investigate the protective effect and mechanism of ligustrazine on cel apoptosis in the acute stage of pulmonary contusion induced by blunt chest trauma in rats. METHODS: Healthy male Sprague-Dawley rats were divided equal y and randomly into three groups: control group, model group and ligustrazine group. Blunt chest trauma models were prepared in the latter two groups. Rats in the ligustrazine group were intraperitoneal y injected with 80 mg/kg ligustrazine immediately after blunt chest trauma. Lung tissues were col ected at 1, 2 and 3 hours after blunt chest trauma to observe pathomorphological changes. The apoptotic index, pulmonary microvascular permeability and severity of pulmonary edema were detected to assess the lung function. Expressions of Caspase-3, Bax and Bcl-2 were detected by immunohistochemical staining, and blood tumor necrosis factor alpha was also detected. RESULTS AND CONCLUSION: In the model group, tumor necrosis factor alpha increased at 1 hour and increased sharply in 2 and 3 hours after blunt chest trauma (P < 0.05); the apoptotic index and the degree of lung injury increased significantly at 2 and 3 hours after blunt chest trauma (P < 0.05); pulmonary microvascular permeability and degree of pulmonary edema were increased (P < 0.05); the expression of Caspase-3 increase significantly (P < 0.05) with the decrease of the ratio of Bcl-2 to Bax (P < 0.05). In the ligustrazine group, tumor necrosis factor alpha decreased notably (P < 0.05); the apoptotic index and the degree of lung injury decreased significantly after blunt chest trauma (P < 0.05); pulmonary microvascular permeability and the degree of pulmonary edema were relieved (P < 0.05); the expression of Caspase-3 decreased significantly (P < 0.05) with the increase of the ratio of Bcl-2 to Bax after treated with ligustrazine (P < 0.01). These findings indicate that ligustrazine can al eviate cel apoptosis in the acute stage of pulmonary contusion induced by blunt chest trauma by inhibiting the tumor necrosis factor alpha, down-regulating the expression of Caspase-3 and enhancing the ratio of Bcl-2/Bax.关键词
组织构建/组织构建基础实验/川芎嗪/凋亡/肺损伤/胸部撞击伤/细胞凋亡指数/肺水肿/肺组织损伤程度/Caspase-3/Bcl-2/Bax/肿瘤坏死因子 α/国家自然科学基金/组织构建图片文章分类
医药卫生引用本文复制引用
曹晨,邓应忠,郑明安,刘芳,孟庆涛..川芎嗪干预钝性肺挫伤急性期大鼠肺组织细胞的凋亡*[J].中国组织工程研究,2013,(11):2048-2053,6.基金项目
国家自然科学基金资助项目(81000027)。Supported by：the National Natural Science Foundation of China, No.81000027* （81000027）
