中国组织工程研究Issue(11)：2083-2090,8.DOI:10.3969/j.issn.2095-4344.2013.11.027
传统中药对成骨细胞及骨钙素合成的影响*★
Traditional Chinese medicine promotes the osteocalcin synthesis of osteoblasts
摘要
Abstract
BACKGROUND: In vitro culture of osteoblasts and its Chinese medicine intervention is one of the important means for the research and development of osteoporosis treated with traditional Chinese medicine. OBJECTIVE: To observe the effect of Chinese medicine on the proliferation and differentiation of osteoblasts and the synthesis of osteocalcin. METHODS: The researches on the effect of different kinds of Chinese medincine-loaded serum on the secretion of osteoblasts and osteocalcin were col ected for the data analysis. The effect of traditional Chinese medicine on osteocalcin synthesis of osteoblasts was observed from the level of molecular cel biology. RESULTS AND CONCLUSION: Some single Chinese herbs and Chinese herbal compounds could promote the proliferation and differentiation of osteoblasts and the synthesis of osteocalcin, and different Chinese medicines had different effects in promoting the proliferation and differentiation of osteoblasts and expression level of osteocalcin, which was positively correlated with the concentration of Chinese medicine-loaded serum. Through screening with the in vitro culture of osteoblasts, the drugs and appropriate dose for promoting the proliferation and differentiation were founded, which could positively promote the development of drugs used for the treatment of osteoporosis.关键词
组织构建/组织构建学术探讨/中药/成骨细胞/骨钙素/骨质疏松/碱性磷酸酶/体外高糖/羧化不全骨钙素/提取液/载药血清/增殖/骨密度/骨形态蛋白/省级基金分类
医药卫生引用本文复制引用
魏双平,李蒙,李瑞玉,吴立萍,孙艳孚,颜军礼..传统中药对成骨细胞及骨钙素合成的影响*★[J].中国组织工程研究,2013,(11):2083-2090,8.基金项目
河北省中医药管理局资助项目(2012068)。Supported by：Projects of Hebei Provincial Administration of Traditional Chinese Medicine, No.2012068* （2012068）
