中国组织工程研究Issue(14)：2471-2479,9.DOI:10.3969/j.issn.2095-4344.2013.14.001
MRI活体示踪心肌梗死大鼠骨髓间充质干细胞的移植***☆
In vivo MRI-traced bone marrow mesenchymal stem cell transplantation in myocardial infarction rats
摘要
Abstract
BACKGROUND: The continuous monitoring of the in vivo survival, distribution, migration, proliferation and differentiation of the stem cel s is important to evaluate the efficacy and safety of stem cel s transplantation. OBJECTIVE: To observe the distribution and migration of superparamagnetic iron oxide labeled bone marrow mesenchymal stem cel s in ischemic myocardial tissue traced with MRI. METHODS: Rat bone marrow mesenchymal stem cel s were separated and cultured by direct attachment method. The surface antigens of the bone marrow mesenchymal stem cel s were identified. The bone marrow mesenchymal stem cel s were labeled by the novel superparamagnetic iron oxide. The feasibility of labeled bone marrow mesenchymal stem cel s was determined by MRI in vitro. Trypan blue exclusion test and methylthiazolyldphenyl-tetrazolium bromide colorimetric test were performed to detect the activity and proliferation of the labeled cel s. A total of 60 Sprague Dawley rats were divided into three groups, and the rats were used to establish the myocardial infarction model. At 2 weeks after modeling, the rats were re-implanted with phosphate-buffered solution containing labeled bone marrow mesenchymal stem cel s, phosphate-buffered solution containing unlabeled bone marrow mesenchymal stem cel s and phosphate-buffered solution in the same dose, respectively. Al rats in each group underwent MRI at 1 day and 3 weeks after transplantation to dynamical y monitor the distribution and migration of transplanted cel s. CD90 immunohistochemistry was done according to MRI. RESULTS AND CONCLUSION: After superparamagnetic iron oxide labeling, the Prussian blue staining showed that the blue iron particles were inside the cytoplasm and the labeling rate was 99%. There was no statistical y difference of trypan blue exclusion rate and methylthiazolyldphenyl-tetrazolium bromide absorption value between labeled and unlabeled cel s. Labeled cel s could be detected as low signal intensity on T2WI and T2W/FFE with in vitro MRI. Labeled bone marrow mesenchymal stem cel s showed round low signal intensity on T2WI, T2W/FFE at the edge of the infarcted myocardium at 1 day after transplantation, and the initial low signal became obscure gradual y, extended and contrast reduced at 3 weeks after transplantation. CD90 immunohistochemistry staining confirmed that transplanted bone marrow mesenchymal stem cel s could migrate from the border to the infarcted region. It was feasible to label the bone marrow mesenchymal stem cel s with the novel superparamagnetic iron oxide in rats and the labeled bone marrow mesenchymal stem cel s could be tracked on MRI in vitro.关键词
干细胞/骨髓干细胞/磁共振成像/心肌梗死/骨髓间充质干细胞/干细胞移植/活体/示踪/大鼠/省级基金/干细胞图片文章Key words
stem cel s/bone marrow-derived stem cel s/magnetic resonance imaging/myocardial infarction/bone marrow mesenchymal stem cel s/stem cel transplantation/in vivo/tracer/rats/provincial grants-supported paper/stem cel photographs-containing paper分类
医药卫生引用本文复制引用
华平,王友于,杨淞然,刘家良,刘立宝,陶俊,杨艳旗..MRI活体示踪心肌梗死大鼠骨髓间充质干细胞的移植***☆[J].中国组织工程研究,2013,(14):2471-2479,9.基金项目
中央高校基本科研业务费专项资金资助；广东省科技计划项目(2012B031800258)；广东省科技计划项目(2007B050200011)。 （2012B031800258）
