中国组织工程研究Issue(14)：2586-2591,6.DOI:10.3969/j.issn.2095-4344.2013.14.017
Turner综合征羊水干细胞的分离及特征*★◆
Isolation and characterization of amniotic fluid-derived stem cells in Turner’s syndrome
摘要
Abstract
BACKGROUND: Great progress has been achieved on studies on isolation, culture and biological characteristics of amniotic fluid-derived mesenchymal stem cel s. However, few studies are reported on amniotic fluid-derived mesenchymal stem cel s in 45, X/46, XX (Turner’s syndrome). OBJECTIVE: To develop a simple culture protocol to isolate amniotic fluid-derived mesenchymal stem cel s and investigate the biological characteristics of amniotic fluid-derived mesenchymal stem cel s. METHODS: We developed a gradient dilution culture protocol to isolate a population of 45, X/46, XX (Turner’s syndrome) amniotic fluid-derived mesenchymal stem cel s from second-trimester amniocentesis. The morphology of amniotic fluid-derived mesenchymal stem cel s was observed by light microscope. The karyotype was analyzed. Specific cel surface antigens and cel cycle of the clonal amniotic fluid-derived mesenchymal stem cel s at passage 4 were characterized by flow cytometry. Osteogenic differentiation of amniotic fluid-derived mesenchymal stem cel s was confirmed by alkaline phosphatase staining and alizarin red staining. RESULTS AND CONCLUSION: The cultured human amniotic fluid-derived mesenchymal stem cel s proliferated rapidly after passage. Karyotype mapping showed abnormal female chromosome type with 45, X/46, XX observed. The amniotic fluid-derived mesenchymal stem cel s had an immunophenotype similar to that of common mesenchymal stem cel s and were positive for CD29, CD44, CD90 and CD105, but negative for CD34 and CD45. The cel cycle measurement showed that amniotic fluid-derived mesenchymal stem cel s cultured in vitro could maintain strong proliferation ability. Alkaline phosphatase staining and alizarin red staining results confirmed that amniotic fluid-derived mesenchymal stem cel s could be successful y induced to differentiate into osteocytes under specific culture media. These results demonstrated that amniotic fluid-derived mesenchymal stem cel s in 45, X/46, XX (Turner’s syndrome) were successful y isolated and the cel s had a great potential of proliferation and showed the characteristics of mesenchymal stem cel s.关键词
干细胞/干细胞培养与分化/Turner 综合征/羊水细胞/羊水干细胞/染色体核型/成骨分化/省级基金/干细胞图片文章Key words
stem cel s/stem cel culture and differentiation/Turner’s syndrome/amniotic fluid cel s/amniotic fluid-derived stem cel s/karyotype/osteogenic differentiation/provincial grants-supported paper/stem cel photographs-containing paper分类
医药卫生引用本文复制引用
龚余,骆玉梅,田霖,刘海波,陈欣洁,孙筱放,陈耀勇..Turner综合征羊水干细胞的分离及特征*★◆[J].中国组织工程研究,2013,(14):2586-2591,6.基金项目
广东省教育厅课题资助(粤财教(2012)290号)。 （粤财教(2012）
