中国组织工程研究Issue(14)：2641-2648,8.DOI:10.3969/j.issn.2095-4344.2013.14.025
骨肉瘤干细胞的分离与鉴定★
Isolation and identification of osteosarcoma stem cells
曲绍政 1李书忠 1高甲科 1张金锋2
作者信息
- 1. 青岛大学医学院附属医院，山东省青岛市 266000
- 2. 青岛市海慈医疗集团，山东省青岛市 266000
- 折叠
摘要
Abstract
BACKGROUND: Osteosarcoma is the most common bone primary malignant tumors, and the occurrence of osteosarcoma may relate with osteosarcoma stem cel s. OBJECTIVE: To evaluate the isolation, culture and identification method of osteosarcoma stem cel s, to investigate the expression of relative tumor markers. METHODS: The osteosarcoma stem cel s were isolated and cultured with magnetic activated cel sorting method under serum-free condition and serum-free joint antineoplastic condition, in order to sort Stro-1 positive and CD133 positive osteosarcoma stem cel s. The expression level and tumorigenic properties of CD133, Oct3 / 4 and Nanog markers of osteosarcoma cancer stem cel s were tested with immunofluorescence staining and Western blotting methods. RESULTS AND CONCLUSION: Suspended cel condensation could be seen in the osteosarcoma stem cel s after culture for 2-10 days, the proliferation incubation period was about 24 hours, and the Stro-1 positive stem cel s could form the suspended cel condensation, while the Stro-1 negative stem cel s could not form the suspended cel condensation. In addition, osteosarcoma stem cel s could highly express Oct3/4, Nanog and CD133, the CD133-positive osteosarcoma stem cel s could highly express CD133 molecule with strong invasiveness, while the CD133 negative cel s could not express CD133 molecule and the invasiveness was weak. Celecoxib could inhibit the formation of osteosarcoma stem cel s to some extent, and could reduce the expression of tumor angiogenic blood vessels and vascular endothelial growth factor.关键词
干细胞/干细胞学术探讨/骨肿瘤/骨肉瘤/肿瘤干细胞/阿霉素/长春新碱/CD133/Nanog/Stro-1/悬浮细胞Key words
stem cel s/stem cel academic discussion/bone tumors/osteosarcoma/tumor stem cel s/doxorubicin/vincristine/CD133/Nanog/Stro-1/suspended cel s分类
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曲绍政,李书忠,高甲科,张金锋..骨肉瘤干细胞的分离与鉴定★[J].中国组织工程研究,2013,(14):2641-2648,8.
