摘要
Abstract
BACKGROUND:At present, there are no systematic pharmacodynamic studies addressing osteoporosis treatment with Chinese medicine. Chinese prescription is not simple enough and research on the mechanism is not close to the guiding of Chinese medicine theory. <br> OBJECTIVE:To observe the therapeutic effects of turtle deer glue, epimedium, and rhodiola pil s on serum indices, bone mineral density and osteoclast regulation in ovariectomized rats. <br> METHODS:Ovariectomized rats were prepared and subjected to intragastric administration of turtle deer glue, epimedium and rhodiola in comparison with sham-surgery and model groups. The body mass was detected once a week starting from week 4. Bone mineral density, serum calcium, phosphorus content, and blood alkaline phosphatase activity were detected at week 12. Osteoclast inhibiting rates were evaluated by in vitro osteoclast culture model. <br> RESULTS AND CONCLUSION:The body mass increased for the model group, the turtle deer glue could significantly reduce the increase rate (P<0.05). After 12 weeks, compared with the model group, the serum calcium content was significantly higher in the three Chinese medicine groups (P<0.05), whereas the blood phosphorus content and alkaline phosphatase activity were obviously lower (P<0.05). Bone mineral density in the turtle deer glue and epimedium groups was significantly increased as compared with that in the model group (P<0.05). The number of resorption pits was decreased significantly in the three Chinese medicine groups, especial y in the rhodiola group, as compared with the sham-surgery group (P<0.05). Rhodiola pil s could prohibit the absorptive function of osteoclasts, the inhibitory rate of which was 86.85%(P<0.01). These findings indicate that turtle deer glue, epimedium, and rhodiola al have significant effects on ovariectomized osteoporosis. Turtle deer glue and epimedium mainly increase bone mineral density, while rhodiola pil s prohibit the absorptive function of osteoclasts.关键词
组织构建/骨组织构建/骨质疏松症/卵巢切除大鼠/龟鹿胶/淫羊藿/红景天/血清/骨密度/破骨细胞/绝经/血磷/血钙/碱性磷酸酶Key words
tissue construction/bone tissue construction/osteoporosis/ovariectomized rats/turtle deer glue/epimedium/rhodiola/serum/bone mineral density/osteoclasts/menopause/blood phosphorus/blood calcium/alkaline phosphatase分类
医药卫生
