中国组织工程研究Issue(15)：2685-2692,8.DOI:10.3969/j.issn.2095-4344.2013.15.004
四种血清生物标志物联合检测早期诊断和评价骨关节炎的价值*★
Early diagnosis value of combination detection of four serum biomarkers for osteoarthritis
摘要
Abstract
BACKGROUND:Studies have shown that biomarkers in the diagnosis of early osteoarthritis have their unique advantages, but so far, it is not clear on application value of biomarkers in the diagnosis of early osteoarthritis in human being. <br> OBJECTIVE:To investigate the value and significance as early diagnosis and condition assessment indicators of osteoarthritis by means of quantitative detection of the changes in C-telopeptide of col agen typeⅡ, matrix metal oproteinase protein-3, tumor necrosis factor-α, and interleukin-1. <br> METHODS:Thirty-five New Zealand white rabbits were divided into experimental group (n=28) and control group (n=7). Resection of the ligament and meniscus was performed in the experimental group for modeling. No treatment was done in the control group. C-telopeptide of col agen type Ⅱ, matrix metal oproteinase protein-3, tumor necrosis factor-α, and interleukin-1 levels were detected at 1, 2, 3, 4 weeks after modeling in the experimental group, and these biomarkers were also detected in the control group at the same time points. Knee MRI examination, joint sample general observation and histopathological observation were conducted. <br> RESULTS AND CONCLUSION:Levels of four biomarkers in the experimental group were significantly higher than those in the control group at each time point after modeling (P<0.05). Histopatological changes and knee MRI variation did not occur at 1 week after operation;however, these changes appeared at 2 weeks, and strengthened at 3-4 weeks. In addition, the levels of four biomarkers began to increase at 1 week after operation. The results suggest that combination detection of C-telopeptide of col agen type II, matrix metal oproteinase protein-3, tumor necrosis factor-α, and interleukin-1 in serum can become a reference indicator for early diagnosis and assessment of osteoarthritis.关键词
组织构建/骨组织构建/骨关节炎/Ⅱ型胶原C-端肽/基质金属蛋白酶3/肿瘤坏死因子α/白细胞介素1/省级基金Key words
tissue construction/bone tissue construction/osteoarthritis/collagen type Ⅱ C-telopeptide/matrix metal oproteinase-3/tumor necrosis factor-alpha/interleukin-1/provincial grants-supported paper分类
医药卫生引用本文复制引用
于伟光,张新潮,朱行飞,刘志勇..四种血清生物标志物联合检测早期诊断和评价骨关节炎的价值*★[J].中国组织工程研究,2013,(15):2685-2692,8.基金项目
上海市卫生局科研计划课题资助项目(2009-34)，课题名称：Ⅱ型胶原C-端肽在兔骨关节炎早期诊断方面的应用价值。 （2009-34）
