中国组织工程研究Issue(15)：2693-2697,5.DOI:10.3969/j.issn.2095-4344.2013.15.005
建立小鼠成骨与破骨细胞相互作用的共育新体系**☆
A novel co-culture system for interaction of mouse osteoblasts and osteoclasts**☆
摘要
Abstract
BACKGROUND:It is wel known that bone remodeling is an important biological process in which osteoblasts and osteoclasts play the critical role. However, the detailed mechanisms of osteoblast-osteoclast communication in bone remodeling remain unclear. <br> OBJECTIVE:To in vitro establish a novel co-culture system for interaction of mouse osteoblasts and osteoclasts using the Transwel technology. <br> METHODS:Osteoblastic cel line MC3T3-E1 and preosteoclasts RAW264.7 were selected, and then the cel s were induced to differentiate into osteoblasts and osteoclasts, respectively. The co-culture system for osteoblasts and osteoclasts interaction was established with Transwel co-culture plate (0.4 µm polyester film), and the osteoblasts and osteoclasts were co-cultured for 6 days. After that, the proliferation and differentiation activities of osteoblasts were analyzed through testing the cel activity and alkaline phosphatase activity, and the differentiation and bone resorption of osteoclasts were observed using tartrate-resistant acid phosphatase staining, toluidine blue staining, tartrate-resistant acid phosphatase activity detecting and scanning microscope technology. <br> RESULTS AND CONCLUSION:In the co-culture system, the unlimited proliferation ability of osteoblasts was decreased, while the differentiation activity was increased. Meanwhile, the preosteoclasts could differentiate into mature osteoclasts with bone resorption function. Thus, this co-culture system can be applied in the further study of osteoblast-osteoclast communication in bone remodeling.关键词
组织构建/骨组织构建/共培养/成骨细胞/破骨细胞/碱性磷酸酶/抗酒石酸酸性磷酸酶/国家自然科学基金Key words
tissue construction/bone tissue construction/co-culture/osteoblasts/osteoclasts/alkaline phosphatase/tartrate-resistant acid phosphatase/National Natural Science Foundation of China分类
医药卫生引用本文复制引用
李建宇,刘璐,万宗明,郝庆新,李瑞欣,郭勇,张西正..建立小鼠成骨与破骨细胞相互作用的共育新体系**☆[J].中国组织工程研究,2013,(15):2693-2697,5.基金项目
国家自然科学重点基金(10832012)；天津市自然科学基金 （10832012）
