中国组织工程研究Issue(15)：2769-2775,7.DOI:10.3969/j.issn.2095-4344.2013.15.016
原代人宫颈癌细胞的培养方法*★
Culture methods for primary cervical carcinoma cells
摘要
Abstract
BACKGROUND:In vitro isolation and culture of cervical epithelial carcinoma cel s is the foundation for studying tumor etiology and treatment from the cel ular level, which is the most basic aspect of cervical cancer tissue engineering research. <br> OBJECTIVE:To investigate the culture methods for primary cervical carcinoma cel s, and passaged cel growth, proliferation and shape in vitro. <br> METHODS:We isolated and cultured primary cervical carcinoma cel by using enzyme joint digestion technique (0.25%trypsin and 2%type I col agenase), observed the shape of the tumor cel and condition of the cel proliferation using the inverted fluorescent microscope, and detected CK17 and Ki67 expression by immunofluorescence. <br> RESULTS AND CONCLUSION:Cervical epithelial carcinoma cel s harvested using the enzyme joint digestion technique had a higher purity, and this technique was relatively simple to be repeated. The passaged cervical epithelial carcinoma cel s also had a stable cel phenotype. CK17 positive expression indicated the cultured cel s were cervical epithelial carcinoma cel s and Ki67 positive expression showed that the cultured cel s had the proliferative characteristics of malignant tumor cel s.关键词
组织构建/组织构建细胞学实验/细胞培养/宫颈/上皮细胞/培养基/胰蛋白酶/Ⅰ型胶原酶/原代细胞/体外培养/生长特点/肿瘤标记物/CK17/Ki67/省级基金Key words
tissue construction/cytology experiment in tissue construction/cell culture/cervix/epithelial cells/medium/trypsin/col agenase type Ⅰ/primary cells/in vitro culture/growth characteristics/tumor markers/CK17/Ki67/provincial grants-supported paper分类
医药卫生引用本文复制引用
程静新,马秀萍,周萍,袁敏,黄坤,张怡..原代人宫颈癌细胞的培养方法*★[J].中国组织工程研究,2013,(15):2769-2775,7.基金项目
新疆维吾尔自治区自然科学基金资助项目(2010211A31)，课题名称：宫颈癌个体化新辅助化疗模式探讨。 （2010211A31）
