中国组织工程研究Issue(16)：2874-2882,9.DOI:10.3969/j.issn.2095-4344.2013.16.004
煅烧骨的安全性*★
Safety of true bone ceramics*★
摘要
Abstract
BACKGROUND:True bone ceramic treated with high temperature has continuous microporous structure, good biological compatibility and degradation, which are analogous to nature bone. <br> OBJECTIVE:To observe the biocompatibility, cel ular compatibility and acute toxicity of implanted composite made from periosteal-derived osteoblasts and sintered bovine bone (true bone ceramics). <br> METHODS:Experiment of cel ular compatibility:bovine true bone ceramics were compound cultured with induced passage 3 bone marrow mesenchymal stem cel s of Wistar rats. Hemolysis test:the true bone ceramic water extract, normal saline and double-distil ed water were added into rabbit blood. Blood coagulation test:the true bone ceramics were added into rabbit blood plasma. Systemic acute toxicity test:true bone ceramic water extract and normal saline were injected into the Kunming mice via the tail vein respectively. Micronucleus test:true bone ceramic water extract, normal saline and cyclophosphamide were given into the mice through intraperitoneal injection respectively. Local irritation reaction:true bone ceramic water extract and normal saline were injected at the two sides of the spine respectively. Pyrogen test:true bone ceramic water extract was injected via the ear vein. The subcutaneous implantation test:the true bone ceramic materials were implanted dorsal subcutaneously. <br> RESULTS AND CONCLUSION:True bone ceramics had no cel toxicity, but had good cel ular and blood compatibility;had no stimulation to muscle and skin;had no toxicity on heart, liver and kidney;had no stimulation to the surrounding tissues after subcutaneous implantation, and part of the materials were degraded and replaced by newly developed fibrous tissue;had no pyrogenic action and had no influence on coagulation;had no inhibition and toxic effect on bone marrow cel s.关键词
生物材料/组织工程骨材料/煅烧骨/细胞相容性/生物相容性/毒性/骨髓间充质干细胞/浸提液/组织工程骨/省级基金Key words
biomaterials/tissue-engineered bone materials/true bone ceramic/cellular compatibility/biocompatibility/toxicity/bone marrow mesenchymal stem cells/water extract/tissue-engineered bone/bone/provincial grants-supported paper分类
医药卫生引用本文复制引用
刘斌钰,郭敏芳,邢雁霞,马存根..煅烧骨的安全性*★[J].中国组织工程研究,2013,(16):2874-2882,9.基金项目
山西省高校科技研究开发项目(20111120)。 （20111120）
