中国组织工程研究Issue(18)：124-132,9.DOI:10.3969/j.issn.2095-4344.2013.18.017
带血管肌筋膜包埋脂肪干细胞载体复合物构建血管化组织工程脂肪***★
Vascularized tissue-engineered adipose established via the adipose-derived stem cells-attached scaffolds encapsulated in muscular fasciae with axial pattern blood vessel pedicle***★
摘要
Abstract
BACKGROUND:Revascularization mechanism is the decisive factor for the successful construction of tissue-engineered adipose tissue. <br> OBJECTIVE:To observe the difference of in vivo adipogenic efficacy of adipose-derived stem cel s and col agen protein scaffold encapsulated in three muscular fasciae. <br> METHODS:The rabbit right vascularized latissimus dorsi muscular fasciae was separated and encapsulated with type Ⅰ col agen protein and adipogenic differentiated adipose-derived stem cel s complexes as the differentiation group with axial pattern blood vessel pedicle. The rabbit left vascularized latissimus dorsi muscular fasciae was separated and encapsulated with type Ⅰ col agen protein and undifferentiated adipose-derived stem cel s complexes as the undifferentiation group with axial pattern blood vessel pedicle. The rabbit gluteus maximus muscular fasciae without specific vascular pedicle was separated and encapsulated with type Ⅰ col agen protein and undifferentiated adipose-derived stem cel s complexes as the control group. <br> RESULTS AND CONCLUSION:Eight weeks after transplantation, hematoxylin-eosin staining and immunohistochemical staining showed that there was new adipose tissue formation in al groups. The mean humid weight of the neonatal fat tissue and the microvessel density in the differentiation group was higher than those in the undifferentiation group and the control group (P<0.01, P<0.05). It indicated that the vascularized tissue-engineered adipose was successful y established via adipose-derived stem cel s-attached scaffolds encapsulated in muscular fasciae with axial pattern blood vessel pedicle with good in vivo adipogenic efficiency and strong ability to promote angiogenesis.关键词
器官移植/组织移植/器官移植临床应用/脂肪干细胞/成脂分化/背阔肌筋膜瓣/臀大肌筋膜瓣/组织工程化脂肪/脂肪组织/脂肪细胞/血管生成/细胞移植/胶原支架/兔/组织工程/省级基金分类
医药卫生引用本文复制引用
王和庚,黎洪棉,崔世恩,徐昆明..带血管肌筋膜包埋脂肪干细胞载体复合物构建血管化组织工程脂肪***★[J].中国组织工程研究,2013,(18):124-132,9.基金项目
课题受中国博士后科学基金(20090450910)，广东省医学科研基金项目(A2011739)和中山市科技计划项目(20113A008)资助。 （20090450910）
