中国组织工程研究Issue(19)：3443-3448,6.DOI:10.3969/j.issn.2095-4344.2013.19.004
骨髓间充质干细胞干预再生障碍性贫血患者树突状细胞的成熟分化★
Bone marrow mesenchymal stem cells intervene maturation and differentiation of dendritic cells in aplastic anemia patients
张乐琴 1肖扬 1蒋祖军 1肖浩文 1李力 1庞妍1
作者信息
- 1. 解放军广州军区广州总医院，广东省广州市 510010
- 折叠
摘要
Abstract
BACKGROUND:There are few reports about the influence of bone marrow mesenchymal stem cel s on T cel proliferation in aplastic anemia patients in China. Besides, no study is reported in China on the topic that whether bone marrow mesenchymal stem cel s affect the T cel proliferation in aplastic anemia patients by dendritic cel regulation. Therefore, its mechanism deserves further investigations. OBJECTIVE:To explore effects of human bone marrow mesenchymal stem cel s on dendritic cel immune regulation in patients with aplastic anemia. METHODS:Human peripheral blood mononuclear cel s-derived dendritic cel s cultured for 5 days were co-cultured with human bone marrow mesenchymal stem cel s at passage 3. Lipopolysaccharide and tumor necrosis factor were added to promote the maturation of dendritic cel s. Flow cytometry was used to detect the expression of dendritic cel surface markers before and after coculture of bone marrow mesenchymal stem cel s and immature and mature dendritic cel s. RESULTS AND CONCLUSION:Under the induction of lipopolysaccharide, prior to and fol owing the coculture of immature dendritic cel s and bone marrow mesenchymal stem cel s, no changes of the expression of CD14, CD1a, CD83 and CD80 were observed (P>0.05). Prior to and fol owing the coculture of mature dendritic cel s and bone marrow mesenchymal stem cel s, the expression of CD14, CD1a, CD83 and CD80 was decreased (P<0.05). Results suggested that bone marrow mesenchymal stem cel s can inhibit the development and maturation of dendritic cel s derived from monocytes of aplastic anemia patients, and exhibit immunoregulatory effects in patients with aplastic anemia.关键词
干细胞/骨髓干细胞/贫血/再生障碍性/骨髓/间充质干细胞/共培养/单个核细胞/树突状细胞/免疫表型/抑制/免疫调节Key words
stem cells/bone marrow-derived stem cells/anemia/aplastic/bone marrow/mesenchymal stem cells/coculture/mononuclear cells/dendritic cells/immunophenotype/inhibition/immunoregulation分类
医药卫生引用本文复制引用
张乐琴,肖扬,蒋祖军,肖浩文,李力,庞妍..骨髓间充质干细胞干预再生障碍性贫血患者树突状细胞的成熟分化★[J].中国组织工程研究,2013,(19):3443-3448,6.
