中国组织工程研究Issue(19)：3455-3460,6.DOI:10.3969/j.issn.2095-4344.2013.19.006
脑胶质瘤中肿瘤干细胞的体外放射敏感性**★○
In vitro radiosensitivity of brain cancer stem cells in brain glioma
摘要
Abstract
BACKGROUND:Radiotherapy effect on of brain glioma is not ideal, and there are various possible factors that influence the therapeutic effect. OBJECTIVE:To investigate the in vitro radiosensitivity of brain cancer stem cel s in brain glioma. METHODS:Glioma cel s were isolated and seeded into the serum-free medium containing growth factors for culture. Glioma cel s from the most active cel site were subcultured to 3-5 generations. Glioma cel vitality was assessed to determine the optimal dose of X-ray radiation. RESULTS AND CONCLUSION:Distinct difference in activities of proliferation could be found among cancer stem cel s from different sites of glioma. X-ray dosages above 8 Gy had notable kil ing effect on brain cancer stem cel s. Different irradiation dosage had different influence on cancer stem cel s.关键词
干细胞/肿瘤干细胞/脑肿瘤干细胞/胶质瘤/生长因子/放射敏感性/放疗/照射/剂量/个体化定位/增殖活力/省级基金Key words
stem cel s/tumor stem cel s/brain cancer stem cel s/glioma/growth factors/radiosensitivity/radiotherapy/irradiation/dose/individual positioning/proliferation activity/provincial grants-supported paper分类
医药卫生引用本文复制引用
赵中甫,樊锐太,杨永,孙剑瑞,胡祥,Eileen Shen,杨波..脑胶质瘤中肿瘤干细胞的体外放射敏感性**★○[J].中国组织工程研究,2013,(19):3455-3460,6.基金项目
河南省科技厅科技攻关项目(112102310173)；郑州大学第一附属医院创新科研团队建设基金项目(TD2011023)。 （112102310173）
