中国组织工程研究Issue(19)：3503-3507,5.DOI:10.3969/j.issn.2095-4344.2013.19.013
大鼠羊膜上皮细胞体外诱导可分化为类神经干细胞*★
Rat amniotic epithelial cells are induced to differentiate into neural-like stem cells in vitro
摘要
Abstract
BACKGROUND:Previous studies have found that Schwann cel s, olfactory ensheathing cel s, neural stem cel s and activated macrophages may repair nerve injuries, but these cel s have some deficiencies, such as source restrictions, tumorigenicity, and al ograft rejection. Amniotic epithelial cel s have no above-mentioned limits, and possess multiple-differentiation potential. After the induction, amniotic epithelial cel s may differentiate into cardiomyocyte-like cel s, neural stem cel s, liver cel s, osteoblasts and chondrocytes. OBJECTIVE:To culture rat amniotic epithelial cel s which can be induced to differentiate into neural-like stem cel s. METHODS:Rat amniotic epithelial cel s were col ected from late pregnancy rats using digestion method. Serum-free medium of nerve stem cel s was used to induce rat amniotic epithelial cel s into neural-like stem cel s. Normal and induced cel s were identified by immunocytochemistry and reverse transcription-PCR methods. RESULTS AND CONCLUSION:The morphological observation showed that amniotic epithelial cel s cultured in the conditioned medium presented with cel body retraction and part of nuclei showed refractive enhancement;dendrite-and axon-like structures appeared, and these processes were woven into a mesh and adhered firmly. Immunocytochemistry results showed that the induced amniotic epithelial cel s had a slightly stronger expression of Nestin and glial fibril ary acidic protein, while the pluripotent markers SSEA-4 and Vimentin expressions were weakened. Reverse transcription-PCR test showed that the induced amniotic epithelial cel s had a higher mRNA expression of Nestin, glial fibril ary acidic protein and microtubule-associated protein, while the mRNA expression of mesodermal smooth muscle cel marker smooth muscle-22αand pluripotent markers Sox-2 and Nanog declined. These findings suggest that cultured rat amniotic epithelial cel s can be induced to differentiate into neural-like stem cel s.关键词
干细胞/干细胞培养与分化/羊膜上皮细胞/诱导/分化/类神经干细胞/巢蛋白/胶质纤维酸性蛋白/微管相关蛋白/973项目/干细胞图片文章分类
医药卫生引用本文复制引用
郭兵,许家军..大鼠羊膜上皮细胞体外诱导可分化为类神经干细胞*★[J].中国组织工程研究,2013,(19):3503-3507,5.基金项目
国家重点基础研究发展计划(973计划，No.  （）
2005CB724302)。 （）
