中国组织工程研究Issue(20)：3793-3800,8.DOI:10.3969/j.issn.2095-4344.2013.20.025
白细胞介素10启动子基因多态性与强直性脊柱炎的易感性*★
Association between interleukin-10 promoter regions gene polymorphisms and susceptibility of ankylosing spondylitis
摘要
Abstract
BACKGROUND: Gene polymorphism in patients with ankylosing spondylitis, is likely to affect cytokine secretion pattern. OBJECTIVE: To investigate the correlation between single nucleotide polymorphisms and haplotypes of interleukin-10 and the susceptibility of ankylosing spondylitis in Han population from Jiaodong Peninsula of China. METHODS: The serum levels of interleukin-10 were measured with an enzyme-linked immunosorbent assay. The single nucleotide polymorphisms at positions -1082A/G, -819C/T and -592C/A in the interleukin gene promoter were analyzed using polymerase chain reaction and restriction fragment length polymorphism. RESULTS AND CONCLUSION: 110 ankylosing spondylitis patients and 120 ethnic-matched healthy controls were included in this study. The serum levels of interleukin-10 in the ankylosing spondylitis group were significantly higher than that in the healthy control group (Z=-10.9, P < 0.001). Single nucleotide polymorphisms analysis showed there were no significant differences in the allelic and genotypic frequencies of -592A/C between the ankylosing spondylitis group and healthy control group. No -1082GG genotype was found in this study. The frequency of -1082G al ele was increased in ankylosing spondylitis group when compared with that in the healthy control group (P=0.047). In a logistic regression analysis, the odds ratio of -1082AG genotype was 1.993 (95% confidence interval, 1.046-3.800, P=0.034) for ankylosing spondylitis. And the odds ratio of -819CC genotype was 3.125 (95% confidence interval, 1.246-7.836, P=0.015) for ankylosing spondylitis. Furthermore, haplotype analysis revealed that GCC haplotype could significantly increase the risk of ankylosing spondylitis when compared with the ATA haplotype (odd ratio=2.19; 95% confidence interval, 1.13-4.26; P=0.02). The results indicate that the gene haplotype of interleukin-10 has relationship with the susceptibility of ankylosing spondylitis in Han population from Jiaodong Peninsula of China.关键词
组织构建/组织构建临床实践/白细胞介素10/基因多态性/强直性脊柱炎/单核苷酸多态性/单体型/细胞因子/汉族/等位基因/基因型/省级基金Key words
tissue construction/tissue construction clinical practice/interleukin-10/gene polymorphism/ankylosing spondylitis/single nucleotide polymorphism/haplotype/cytokines/Han population/alleles/genotype/provincial grants-supported paper分类
医药卫生引用本文复制引用
申成凯,吕成昱,王英振,张海宁,隋爱华,孟飞..白细胞介素10启动子基因多态性与强直性脊柱炎的易感性*★[J].中国组织工程研究,2013,(20):3793-3800,8.基金项目
山东省科学技术基金主要项目的部分支持(2008GG30002034)。 （2008GG30002034）
