中国组织工程研究Issue(24)：4473-4480,8.DOI:10.3969/j.issn.2095-4344.2013.24.015
冠状静脉逆行灌注碱性成纤维细胞生长因子的体内浓度梯度***★
In vivo concentration gradient of basic fibroblast growth factor after coronary venous retrograde perfusion
摘要
Abstract
BACKGROUND: In vitro studies have demonstrated that the concentration gradient of basic fibroblast growth factor can promote the migration and proliferation of stem cel s. However, it is unclear whether in vivo basic fibroblast growth factor concentration gradient can be established by coronary venous retrograde perfusion. OBJECTIVE: To evaluate the safety of coronary venous retrograde perfusion and establish the concentration gradient of basic fibroblast growth factor between coronary venous blood and target myocardium, and to investigate the time window of basic fibroblast growth factor concentration gradient after coronary venous retrograde perfusion. METHODS: The acute myocardial infarction models were established with ligating method, and 1 week later, coronary venous retrograde perfusion of basic fibroblast growth factor was performed. After perfusion, the time of bal oon dilation was 0, 5, 10 and 15 minutes. The animals were sacrificed immediately after bal oon inflation lifting, and then the enzyme-linked immunosorbent assay was used to measure the concentration of basic fibroblast growth factor in the plasma, farcted myocardium and homogenates of myocardium in the infarct border zone. The basic fibroblast growth factor concentration gradient between the coronary venous blood and infarcted myocardium and myocardium in the infarct border zone was in vivo evaluated. RESULTS AND CONCLUSION: The success rate of coronary venous retrograde perfusion was 100%. No death, cardiac tamponade, or ventricular arrthythmia occurred. There was no significant difference in concentration of basic fibroblast growth factor between coronary venous blood and infarcted myocardium at 5 and 10 minutes after perfusion, while the concentration of basic fibroblast growth factor in the infarcted myocardium was higher than that in the coronary venous blood and myocardium in the infarct border zone. There was no significant difference in the concentration between two groups after bal oon dilation for 15 minutes. The results indicate that after coronary venous retrograde perfusion of basic fibroblast growth factor, the time of bal oon dilation was 5-10 minutes which can establish stable basic fibroblast growth factor concentration gradient between the coronary venous blood and infarcted myocardium, and the concentration is highest in the infarcted myocardium. The stem cel infusion in this time window can enhance its transitional vitality.关键词
组织构建/组织构建与生物活性因子/缺血性心脏病/冠状静脉逆行灌注/生长因子/干细胞/在体/浓度梯度/碱性成纤维细胞生长因子/骨髓间充质干细胞/国家自然科学基金分类
医药卫生引用本文复制引用
甄雷,聂绍平,王晓,缪黄泰,乔世斌,吴星欣,乔岩,刘百球,刘新民,阙斌..冠状静脉逆行灌注碱性成纤维细胞生长因子的体内浓度梯度***★[J].中国组织工程研究,2013,(24):4473-4480,8.基金项目
本文受国家自然科学基金面上项目(81070166，81270284)以及北京市教育委员会科技发展计划面上项目(KM201010025020)资助。 （81070166，81270284）
