中国组织工程研究Issue(25)：4629-4636,8.DOI:10.3969/j.issn.2095-4344.2013.25.010
深低温冻存羟基磷灰石/骨髓间充质干细胞修复兔桡骨缺损*★
Combination of cryopreserved hydroxyapatite/bone marrow mesenchymal stem cells repairs rabbit radial defects
摘要
Abstract
BACKGROUND: Previous studies have showed that hydroxyapatite combined with bone marrow mesenchymal stem cel s has a good effect on bone defects, but the repair effect of cryopreserved composite material is unclear. OBJECTIVE: To observe the effect of cryopreserved hydroxyapatite/bone marrow mesenchymal stem cel s on bone defects. METHODS: Twenty-seven Japanese rabbits were used to prepare 10 mm radial defect models and then randomly divided into cryopreserved composite group, fresh composite group and hydroxyapatite group. The three groups were respectively implanted with cryopreserved hydroxyapatite/bone marrow mesenchymal stem cel s which were cryopreserved at -80℃ for 3 m onths, fresh hydroxyapatite/bone m arrow m esenchym al stem cel s and pure hydroxyapatite. After 8 and 12 weeks, general observation, X-ray observation and hematoxylin-eosin staining were performed, and biomechanical test was done at 12 weeks. RESULTS AND CONCLUSION: At postoperative 12 weeks, bone defects were almost healed in the cryopreserved composite group and fresh composite group. Mature trabecular bone was seen, some canals were of patency, and good shape was found. While in the hydroxyapatite group, cal us formation was less, only part of bone defects were healed, shaping was poor, and new bone formation was also less than the other two groups (P < 0.05). In addition, the maximal loading in the cryopreserved composite group and fresh composite group was significantly higher than that in the hydroxyapatite group (P < 0.05). These findings indicate that the cryopreserved hydroxyapatite/bone marrow mesenchymal stem cel s composite material is similar to the fresh one in the repair capacity.关键词
生物材料/骨组织构建/羟基磷灰石/骨髓间充质干细胞/支架材料复合物/低温冻存/桡骨缺损/省级基金Key words
biomaterials/bone tissue construction/hydroxyapatite/bone marrow mesenchymal stem cel s/composite scaffold materials/cryopreservation/radial defects/provincial grants-supported paper分类
医药卫生引用本文复制引用
邢志远,张继波,孔令菊,刘建生,郑德宇..深低温冻存羟基磷灰石/骨髓间充质干细胞修复兔桡骨缺损*★[J].中国组织工程研究,2013,(25):4629-4636,8.基金项目
实验获得辽宁省教育厅一般项目的支持(L2011144)。 （L2011144）
