中国组织工程研究Issue(27)：4992-4997,6.DOI:10.3969/j.issn.2095-4344.2013.27.008
不同部位脂肪源性干细胞的生物学特性比较☆
Comparison of biological characteristics of adipose-derived stem cells from different parts
摘要
Abstract
BACKGROUND: Whether the differences exist between adipose-derived stem cells isolated from different parts of rats when cultured in vitro has been poorly understood. OBJECTIVE: To compare the growth characteristics and adipogenic ability of adipose-derived stem cells isolated from different parts of rats. METHODS: Freshly isolated adipose-derived stem cells were obtained from 5 mL inguinal groove and greater omentum adipose tissue of F344 rats using type Ⅰ col agenase digestion method. Then, adipose-derived stem cells were counted and cultured in vitro. Morphological and growth characteristics of adipose-derived stem cells derived from the two sites were observed. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was utilized to examine the doubling time of adipose-derived stem cells from different parts. The passage 2 adipose-derived stem cells were induced adipogenical y. Fourteen days after being induced, the differentiated cells were stained with oil red O and the positive cells were counted. The adipogenic differentiation ability of adipose-derived stem cells from the different parts was assessed. RESULTS AND CONCLUSION: (1) The number of adipose-derived stem cells from the greater omentum fat tissue in the same group was (281±10)×107/L, which was significantly higher than that from the inguinal groove fat tissue [(85±10)×107/L] (P < 0.01). Adipose-derived stem cells from the greater omentum and inguinal groove fat tissue achieved the exponential growth period on days 5 and 6, respectively, and achieved the platform period on days 9 and 10, respectively. The corresponding doubling time was 50 hours and 60 hours, respectively. After being passaged, adipose-derived stem cells grew in fibroblast-like shape actively. The adipogenic differentiation rate of adipose-derived stem cells from the greater omentum fat tissue was higher than that from the inguinal groove fat tissue [(38.90±2.86)% vs. (35.30±3.29)%, P < 0.01]. This shows that the number and the adipogenic differentiation ability of adipose-derived stem cells from different parts of the same F344 rat are different.关键词
干细胞/脂肪干细胞/腹股沟/腹腔大网膜/脂肪组织/不同部位/差异/生物学特性/大鼠/细胞分化/种子细胞/干细胞图片文章Key words
stem cels/adipose-derived stem cells/inguinal groove/greater omentum/adipose tissue/different parts/difference/biological characteristics/rats/cell differentiation/seed cells/stem cell photographs-containing paper分类
医药卫生引用本文复制引用
林立新,黄勇,王玉婷,王鹏,王学明,姜蕾,林冠妤..不同部位脂肪源性干细胞的生物学特性比较☆[J].中国组织工程研究,2013,(27):4992-4997,6.基金项目
Supported by：Science &Technology Development Project of Yantai, No.2009155-9* （）
