中国组织工程研究Issue(32)：5749-5756,8.DOI:10.3969/j.issn.2095-4344.2013.32.002
骨髓间充质干细胞参与A549肺腺癌的组织修复★
Bone marrow mesenchymal stem cells are involved in tissue repair of A549 lung adenocarcinoma
许峰 1张雷 2潘晋坤 2薛利利 2赵晓燕 2李宝平2
作者信息
- 1. 南通市肿瘤医院胸外科，江苏省南通市 226361
- 2. 山西医科大学第二医院肿瘤生物科，山西省太原市 030001
- 折叠
摘要
Abstract
BACKGROUND:Tumor has been considered as a specific nonhealing trauma. Bone marrow mesenchymal stem cel s participate in tumor mesenchymal reconstitution by tumor tissue homing and differentiation into mesenchyme, resulting in changing tumor microenvironment and affecting tumor growth and transfer. OBJECTIVE:To explore the mechanisms of participation of bone marrow mesenchymal stem cel s in tumor tissue repair in an A549 lung cancer-bearing mouse model. METHODS:Bone marrow mesenchymal stem cel s were isolated in vitro, cultured, and identified using flow cytometry, and then used to establish a mouse model of A549 lung cancer-bearing. In the experimental group, human bone marrow mesenchymal stem cel s were injected into tissue surrounding the tumor. In the control group, an equal volume of PBS was injected. Animal survival condition and tumor size were compared. At 4 weeks, the specimens were harvested. Hematoxylin-eosin staining was used to compare tumor tissue. Masson staining was utilized to compare col agen fiber content. Reverse transcription-PCR was employed to detect the expression ofα-smooth muscle actin. Immunohistochemistry was used to examine the expression of fibroblast specific protein and fibroblast activation protein to reflect the degree of interstitial fibers in tumor tissue in both groups. The expression levels of vascular endothelial growth factor, hepatocyte growth factor, interleukin-6 and tenescin-C were compared between the two groups using immunohistochemistry. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s promoted tumor growth in tumor-bearing mice. The growth rate of tumor tissue in experimental group was faster than the control group (P<0.05). Compared with the control group,α-smooth muscle actin mRNA expression was significantly higher in the experimental group. Immunohistochemistry was used to detect the expression of tumor angiogenesis factors markers (fibroblast specific protein and fibroblast activation protein) in tumor tissue of experimental group. The expression levels of vascular endothelial growth factor, hepatocyte growth factor, interleukin-6 and tenescin-C were significantly greater in the experimental group than in the control group (P<0.05). Results indicated that bone marrow mesenchymal stem cel s differentiated into fibroblasts in tumor microenvironment, participated in the formation and construction of tumor stroma as wel as promoted the growth and repair of tumor via the secretion of vascular endothelial growth factor, hepatocyte growth factor, interleukin-6 and tenescin-C.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/A549荷瘤小鼠肺癌/肿瘤相关成纤维细胞/间质纤维/免疫组化/α平滑肌收缩蛋白/管内皮生长因子/肝细胞生长因子/白细胞介素6/肌糖蛋白C/干细胞图片文章分类
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许峰,张雷,潘晋坤,薛利利,赵晓燕,李宝平..骨髓间充质干细胞参与A549肺腺癌的组织修复★[J].中国组织工程研究,2013,(32):5749-5756,8.
