中国组织工程研究Issue(32)：5799-5805,7.DOI:10.3969/j.issn.2095-4344.2013.32.009
人参皂苷Rb1对体外培养人脂肪干细胞增殖与成骨分化的影响**★
Ginsenoside Rb1 affects the proliferation and osteogenic differentiation of human adipose-derived stem cells in vitro**★
摘要
Abstract
BACKGROUND:Various factors can affect the osteogenic differentiation of human adipose-derived stem cel s, and the osteoinductive factor of traditional Chinese medicine is very important for the research of human adipose-derived stem cel s. OBJECTIVE:To investagate the effects of ginsenoside Rb1 on the proliferation and osteogenic differentiation of human adipose-derived stem cel s in vitro. METHODS:The human adipose-derived stem cel s were isolated and cultured in vitro. After passaqed to the third generation, human adipose-derived stem cel s at 2×103/wel were incubated in a 96-wel plate, and treated with 200μL of 0.5, 1.0, 2.0, 4.0,6.0μmol/L ginsenoside Rb1 medium. The human adipose-derived stem cel s in the control group were treated with an equal volume of Dulbecco’s modified Eagle medium. Growth curves were examined by 2,3-bis-(2-methoxy-4-nitro-5-sulphenyl)-(2H)-tetrazolium-5-carboxanilide T colorimetric assay. Alkaline phosphatase activity and osteocalcin content were detected by alkaline phosphatase kit and radio-immunity method, respectively. Calcified nodules were observed using alizarin red O staining. RESULTS AND CONCLUSION:The proliferation viability of human adipose-derived stem cel s was significantly increased after cultured with 0.5μmol/L ginsenoside Rb1. With the increasing of the concentration of ginsenoside Rb1, the mitogenic activity of the cel s was decreased. The 6.0μmol/L ginsenoside Rb1 showed a depressant effect on proliferation. Ginsenoside Rb1 could promote alkaline phosphatase activity and osteocalcin expression in human adipose-derived stem cel s and showed a dose-dependent manner. Calcified nodule formation induced by 4.6 and 6.0μmol/L ginsenoside Rb1 were better when compared with 0.5, 1.0 and 2.0μmol/L ginsenoside Rb1. Ginsenoside Rb1 can promote the proliferation of human adipose-derived stem cel s cultured in vitro in a certain concentration, and in the high concentration, the ginsenoside Rb1 can promote the osteogenic differentiation of human adipose-derived stem cel s. So ginsenoside Rb1 can be used as an osteoinductive factor.关键词
干细胞/脂肪干细胞/人参皂苷/肝细胞/细胞增殖/成骨分化/中药/碱性磷酸酶/骨钙素/钙化结节/省级基金/干细胞图片文章分类
医药卫生引用本文复制引用
罗志军,黎洪棉,王和庚,柳大烈,南华..人参皂苷Rb1对体外培养人脂肪干细胞增殖与成骨分化的影响**★[J].中国组织工程研究,2013,(32):5799-5805,7.基金项目
广东省医学科研课题(A2011739)；中山市科技计划项目(20113A008)。Supported by：Medical Research Project of Guangdong Province, No. A2011739* （A2011739）
Zhongshan Municipal Science and Technology Planning Project, No.20113A008* （）
