中国组织工程研究Issue(33)：5909-5916,8.DOI:10.3969/j.issn.2095-4344.2013.33.002
长距离跑步大鼠膝关节滑膜基质金属蛋白酶的表达***
Expression of matrix metalloproteinases in the synovium of rat knee joint during long-distance running
摘要
Abstract
BACKGROUND:Many studies have shown that matrix metal oproteinases 1, 3, 9 and 13 play an important role in articular cartilage degeneration and destruction, but there is less special research on the articular synovium. OBJECTIVE:To observe the effect of long-distance running on the expressions of matrix metal oproteinases 1, 3, 9 and 13 in the synovium. <br> METHODS:Fifteen male Wistar rats were divided into three groups:control group, tablet group and uphil group. Rats in the control group received ordinary captivity;rats in the tablet group ran on the horizontal treadmil (0°) at the speed of 1 km/h for 1 hour daily, and lasted for 45 days;rats in the uphil group daily ran on the horizontal <br> treadmil (0°) at the speed of 1 km/h for 1 hour, and lasted for 15 days, and then the rats ran on the uphil treadmil (+20°) at the speed of 1 km/h for 1 hour daily and lasted for 30 days. The knee joint synovium injury models with varying degrees were established. The dual hind knee joints were obtained after modeling for paraffin-embedded. Then the overal sagittal slices were obtained for hematoxylin-eosin staining and immunohistochemical staining, and the experimental results were observed and analyzed. <br> RESULTS AND CONCLUSION:After long-distance running, the expression of matrix metal oproteinases 1 in synovium of the tablet group and uphil group was increased when compared with that of the control group (P<0.05), but there was no significant difference between tablet group and uphil group (P>0.05). There was no significant difference in matrix metal oproteinases 3 expression (P>0.05). The expressions of matrix metal oproteinase 9 and <br> matrix metal oproteinase 13 in synovium were in gradient increasing state (P<0.05), which were lowest in the control group, increased in the tablet group and highest in the uphil group. The results indicate that long-distance running <br> exercise can influence the normal physiological structure of rat knee joint synovium by changing the expression of matrix metal oproteinases.关键词
组织构建/软骨组织构建/滑膜炎/膝关节/滑膜损伤/基质金属蛋白酶/长距离/跑步运动/免疫组织化学/国家自然科学基金Key words
tissue construction/cartilage tissue construction/synovitis/knee joint/synovial injury/matrix metal oproteinase/long-distance/running exercise/immunohistochemistry/National Natural Science Foundation of China分类
医药卫生引用本文复制引用
上官雅娴,卫小春,李凯,卫建平..长距离跑步大鼠膝关节滑膜基质金属蛋白酶的表达***[J].中国组织工程研究,2013,(33):5909-5916,8.基金项目
National Natural Science Foundation of China, No.81000815* （）
Natural Science Foundation of Shanxi Province, No.2010021034-5* （）
Key Scientific and Technological Planning Projects of Shanxi Province Health Department, No.2011036* （）
