中国组织工程研究Issue(36)：5741-5745,5.DOI:10.3969/j.issn.2095-4344.2015.36.001
SPIO、DAPI双重标记猕猴骨髓间充质干细胞：对细胞活性及增殖的影响
SPIO and DAPI double labeled bone marrow mesenchymal stem cells of macaques:effects on cell viability and proliferation
摘要
Abstract
BACKGROUND:Traditional cel transplantation tracer methods require histological analysis and identification in vitro, which limits the clinical application of stem cel transplantation. So it is urgent to establish an in vivo noninvasive and repeatable tracer method. OBJECTIVE:To observe the effect of SPIO and DAPI double labeling on survival and proliferation of bone marrow mesenchymal stem cel s from macaques. METHODS:Bone marrow mesenchymal stem cel s were derived from bone marrow aspirates of healthy macaques using whole bone marrow adherence method. Then, the cel s were identified using flow cytometry detection. Bone marrow mesenchymal stem cel s were labeled using SPIO and DAPI. Fluorescent microscope was used to detect DAPI positive rate, and Prussian blue staining and transmission electron microscope were employed to measure SPIO positive rate. MTT assay was used to detect cel viability and proliferation. RESULTS AND CONCLUSION:Bone marrow mesenchymal stem cel s were successful y isolated from healthy macaques using the whole bone marrow adherence method, and the cel purity was up to 95.1%. SPIO and DAPI were both successful to label the bone marrow mesenchymal stem cel s with a positive rate of 95%-98%, but had no influence on cel viability and proliferation.关键词
干细胞/骨髓干细胞/细胞标记/骨髓间充质干细胞/猕猴/SPIO/DAPI/国家自然科学基金分类
医药卫生引用本文复制引用
宋巧巧,周慧良,潘兴华..SPIO、DAPI双重标记猕猴骨髓间充质干细胞：对细胞活性及增殖的影响[J].中国组织工程研究,2015,(36):5741-5745,5.基金项目
国家自然科学基金资助项目(31172170) （31172170）
