中国组织工程研究Issue(40)：6498-6503,6.DOI:10.3969/j.issn.2095-4344.2015.40.019
慢性脑缺血模型大鼠神经突触可塑性与N-甲基-D-天门冬氨酸型受体
The regulating effect of N-methyl-D-aspartate receptor on neural synaptic plasticity in a rat model of chronic cerebral ischemia
摘要
Abstract
BACKGROUND:N-methyl-D-aspartate receptor is an ionic glutamate receptor which is closely related with the neural synaptic plasticity, and also can regulate neural synaptic plasticity. OBJECTIVE:To explore the mechanism by which N-methyl-D-aspartate receptor subunits NR2A and NR2B regulate neural synaptic plasticity after cerebral ischemia. METHODS: 60 Wister rats were randomly and evenly divided into a sham-operated group and a cerebral ischemia group. Rat models of chronic cerebral ischemia were established using the modified bilateral common carotid artery occlusion method in the cerebral ischemia group, while rats in the sham-operated group did not undergo occlusion of the common carotid artery and vagus nerve. RESULTS AND CONCLUSION:At 0-12 hours after chronic cerebral ischemia, NR2A expression in the rat hippocampus was gradualy decreased, while the expression of NR2B reached its peak level at 4 hours after cerebral ischemia. Under the circumstance of cerebral ischemia, neither low frequency nor high frequency induced long-term potentiation. These findings suggest that NR2B exhibit inhibitory effect, while NR2A exhibit promoting effect on long-term potentiation induced by stimulation.关键词
实验动物/脑及脊髓损伤动物模型/慢性脑缺血/N-甲基-D-天门冬氨酸型受体亚单位2A/N-甲基-D-天门冬氨酸型受体亚单位2B/长时程增强/神经突触可塑性/海马CA1区分类
医药卫生引用本文复制引用
王璐,霍帅,王亚飞,赵琳,刘伯锋,阮彩莲,惠雪枫..慢性脑缺血模型大鼠神经突触可塑性与N-甲基-D-天门冬氨酸型受体[J].中国组织工程研究,2015,(40):6498-6503,6.基金项目
陕西省计生委卫生科研项目(2014D30)；延安市2013年度科学技术研究发展计划项目(2013-kw33)@@@@This study was supported by a grant from the Medical and Scientific Research Project of Shaanxi Provincial Family Planning Commission of China, No.2014D30 （2014D30）
a grant from Science and Technology Research Development Program in 2013 of Yanan City in China, No.2013-kw33 （）
