中国组织工程研究Issue(41)：6590-6594,5.DOI:10.3969/j.issn.2095-4344.2015.41.006
PTEN基因沉默对骨髓间充质干细胞生物学特性的影响
PTEN silencing effects on biological properties of bone marrow mesenchymal stem cells
摘要
Abstract
BACKGROUND:The self-renew and regeneration capacity of the injured spinal cord is thought to be limited. Accordingly, cel transplantation is one potential strategy for promoting functional recovery after spinal cord injury. OBJECTIVE:To explore the effects ofPTEN silencing on the biological properties of bone marrow mesenchymal stem cels, hoping to offer better seed cels for tissue engineering. METHODS:Bone marrow mesenchymal stem cels were transfected with specific siRNA-silencedPTEN gene using the liposome method, and then RT-PCR was used to detect the mRNA expression ofPTEN. Variation of biological properties ofPTEN-transfected cels were detected by the way of MTT assay, cel cycle analysis, and Transwel assay. RESULTS AND CONCLUSION:PTEN is expressed highly in bone marrow mesenchymal stem cels, which is successfuly interfered by siRNA.PTEN-silenced cels have stronger survival, proliferation and migration abilities, which become a kind of better seed cels for tissue engineering.关键词
干细胞/骨髓干细胞/骨髓间充质干细胞/PTEN基因/RNA干扰/国家自然科学基金Key words
Bone Marrow/Mesenchymal Stem Cels/PTEN Phosphohydrolase/RNA Interference/Tissue Engineering分类
医药卫生引用本文复制引用
陈毅,陆晓东,刘丹平,屠冠军..PTEN基因沉默对骨髓间充质干细胞生物学特性的影响[J].中国组织工程研究,2015,(41):6590-6594,5.基金项目
国家自然科学基金资助项目(81070971)Funding：the National Natural Science Foundation of China, No.81070971 （81070971）
