中国组织工程研究Issue(42)：6798-6802,5.DOI:10.3969/j.issn.2095-4344.2015.42.014
晶状体胚胎发育过程中促红细胞生成素的作用
Significance of erythropoietin for the embryonic development of the lens
摘要
Abstract
BACKGROUND:Studies have found that erythropoietin has a protective effect on embryonic development of the retina, but there are rare studies concerning the embryonic development of the lens. OBJECTIVE: To observe the difference in erythropoietin expression in the lens from Wistar rats at different embryonic periods and to investigate the effect and significance of erythropoietin in the embryonic development of the lens. METHODS:Clean Wistar rats with pregnancy for 10 days (n=5), 12 days (n=5), 14 days (n=5), 16 days (n=5), 18 days (n=5), 20 days (n=5) were randomly colected and divided into six groups. Every two embryonic ratsof the different 30 pregnant rats were obtained randomly by the caesarean operation under ketamine-induced anesthesia. The eye tissues of al the embryonic rats were isolated and cut into sections. The expression of erythropoietin protein and mRNA in rat lens was detected by immunohistochemistry and reverse t ranscription-PCR, respectively. RESULTS AND CONCLUSION:Erythropoietin was distinctly expressed at the six different embryo stages, and the expression of erythropoietin protein and mRNA gradualy increased from embryonic day 10 to embryonic day 16, and decreased from embryonic day 10 to embryonic day 20. There were significant differences between the six groups. These findings indicate that the expression of embryonic appears in a low to high to low fashion during the embryonic development of Wistar rats, which may be closely associated with the developing procedure of lens.关键词
组织构建/组织工程/促红细胞生成素/晶状体/胚胎发育分类
医药卫生引用本文复制引用
郭庆敏,孟旭霞,田椰,胡迭..晶状体胚胎发育过程中促红细胞生成素的作用[J].中国组织工程研究,2015,(42):6798-6802,5.基金项目
青岛市公共领域科技支撑计划资助项目(09-1-1-18-nsh)Funding：the Public Domain Technology Support Program of Qingdao City, No.09-1-1-18-nsh （09-1-1-18-nsh）
