现代医药卫生Issue(19)：2892-2895,4.DOI:10.3969/j.issn.1009-5519.2015.19.002
8-硝基白杨素诱导人肝癌HepG2细胞分化研究
Study on BrMC induced differentiation of human hepatocarcinoma HepG2 cells
摘要
Abstract
Objective To evaluate whether the artificial semisynthetic analogue 8-bromo-7-methoxychrysin (BrMC), i. e., 8-nitrochrysin as an effective component of traditional Chinese medicine propolis,having the ability for inducing the differen-tiation of human hepatocarcinoma cells. Methods The human hepatocarcinoma HepG2 cells were cultured in vitro. The Wright s-Giemse mixed coloring method was adopted to detect the changes of the cellular morphology and the nuclear-cytoplasmic ratio of Hep G2 cells induced by BrMc;the changes of microtubules microfilament protein array of HepG2 cells induced by BrMC and all trans retinoid acid(ATRA) were observed by Coomassie brilliant blue staining;the contents of alkaline phosphatase(ALP) and gamma-glutamyl transpeptidase(γ-GT) in HepG2 cells were detected by the enzyme catalyzed reaction;the radioimmunoassay(RIA) was used to detect the mass fraction of alpha fetal protein(AFP);the content of tyrosine transaminase(TAT) was measured by the Dia-mondstone spectrophotometry. Results The cytoplasmic ratio by 1.0 ug/mL BrMC handled was far lower than that of solvent con trol group,the difference was statistically significant(P<0.01).1.0μg/mL BrMC and ATRA handled 24,48,96 hours,the mass fraction of AFP,activity ofγ-GT,ALP,TAT compared with the solvent control group respectively,the difference were statistically significant(P<0.01). Conclusion BrMC induces human hepatocarcinoma cellular differentiation by lowing the nucleus-cytoplas-mic ratio,decreasing the secretion of AFP,effectively activating TAT and ALP in HepG2 cells;moreover BrMc and ATRA inhibit-ing the activity ofγ-GT.关键词
植物提取物/肝肿瘤/病理学/肿瘤细胞,培养的/8-硝基白杨素/HepG2细胞/分化/诱导Key words
Plant extracts/Liver neoplasms/pathology/Tumor cells,cultured/8-bromo-7-methoxychrysin(BrMC)/HepG2 cells/Differentiation/Induce引用本文复制引用
何贵成,严思佳,丁恩,艾小红..8-硝基白杨素诱导人肝癌HepG2细胞分化研究[J].现代医药卫生,2015,(19):2892-2895,4.基金项目
湖南省中医药管理局项目（201140）。 （）
