西南林业大学学报Issue(6):1-5,5.DOI:10.11929/j.issn.2095-1914.2015.06.001
盐胁迫下 TheIF1A 基因启动子表达特性分析
The Expression Pattern of TheIF1A Promoter Under Salt Stress
摘要
Abstract
Eukaryotic translation initiation factor 1A in Tamarix hispida (TheIF1A)invovled in the regulation to abiotic stress.In this study,the TheIF1A promoter expression patterns were analyzed under salt stress to explore the genes functions under stress and then,GUS activities among different concentration NaCl and various stress times in the transgenic TheIF1A promoter T4 Arabidopsis were compared.The results showed that TheIF1A promot-er could quickly response to NaCl with 400 mmol /L.The GUS activity was decreased after 15 min treated by NaCl stress.And the highest GUS activities were showed in leaves.The expression of TheIF1A promoter was also obvi-ously decreased under 100 mmol /L NaCl stress.Then the GUS activities were increased.After treated by 20 h,it was obvious higher than 0 h treatment.Among the 3 organs (roots,leaves and petioles),the GUS activity in root was the lowest.These results indicated that the expressions of TheIF1A promoter could response to salt stress,while the expression patters were difference between high and low concentration NaCl stresses.关键词
刚毛柽柳/盐胁迫/表达模式/TheIF1A 启动子Key words
Tamarix hispida/salt stress/expression pattern/TheIF1A promoter分类
农业科技引用本文复制引用
郭宇聪,杨桂燕,赵玉琳,张凤娇,高彩球..盐胁迫下 TheIF1A 基因启动子表达特性分析[J].西南林业大学学报,2015,(6):1-5,5.基金项目
国家自然科学基金项目(31370676)资助;中央高校基本科研业务费专项资金项目(2572014DA03)资助。 ()