西南林业大学学报Issue(6)：14-18,5.DOI:10.11929/j.issn.2095-1914.2015.06.003
橡胶树 Lhcb2基因的克隆与表达特性分析
Molecular Cloning and Expression Analysis of an Lhcb2 Gene from Hevea brasiliensis
摘要
Abstract
Based on the gene sequence identified from the rubber tree (Hevea brasiliensis)genome,an 849 bp cDNA containing an ORF of 798 bp was isolated from the leaf tissue by using the RT-PCR technique. The gene denoted HbLhcb2.1 was predicted to encode 265 amino acids with a theoretical molecular weight of 28.66 kD and isolectric point of 5.42;the protein was promised to be a thylakoid protein of chloroplast;the protein contains one conserved light-harvesting chlorophyll a /b binding protein domain,three transmembrane helices and two C-terminal helices,a trimerization motif,several chlorophyll and carotenoid binding sites, which could be grouped into the LHCB2 subfamily based on the evolutionary relationship.Homologous analysis showed that HbLhcb2.1 shared an identity of about 95 % with the homologs in Ricinus communis,Gossypium hirsutum,Vitis vinifera,Fragaria vesca or Theobroma cacao,suggesting a highly conserved evolution.Expres-sion analysis indicated that HbLhcb2.1 was mainly expressed in the leaves,with a gradual increase as along with the leaf maturity,but a sharp decrease in senescing leaves.关键词
橡胶树/叶绿素a/b结合蛋白/Lhcb2Key words
rubber tree (Hevea brasiliensis)/chlorophyll a/b binding protein/Lhcb2分类
农业科技引用本文复制引用
邹智,王丹华,戴雪梅,朱家立,谢贵水..橡胶树 Lhcb2基因的克隆与表达特性分析[J].西南林业大学学报,2015,(6):14-18,5.基金项目
国家自然科学基金项目（31371556）资助；海南省自然科学基金项目（312026）资助；现代农业产业技术体系建设专项资金项目（CARS －34－GW5）资助；中国热带农业科学院橡胶研究所基本科研业务费专项（1630022015004）资助。 （）
