刑事技术Issue(5)：379-381,3.DOI:10.16467/j.1008-3650.2015.05.008
GlobalFiler™试剂盒在尸骨鉴定中的应用
Identification of Unknown Corpses Using GlobalFiler™ Kit
摘要
Abstract
Objective To analyze the performance of GlobalFiler™ kit testing bones of corpses. Methods Silicon bead method was used to extract DNA from 50 samples of bones and teeth that were all required to determine their source with 11 of them waiting for the triplet and the rest 39 ones the duo paternity testing. The GlobalFiler™ kit and Identifiler Plus kit were employed to evaluate their testing competence and cumulative paternity index (CPI) values. Results GlobalFiler™ kit successfully detected the STR types of the 50 samples. For identification of cadaveric source of the 11 triplet cases, although both kits showed to have CPI values greater than 10,000, the GlobalFiler™ kit performed even better with its CPI larger than 108 in 10 out of the 11 cases, much higher than that of Identifiler Plus kit. For the 39 duo cases, GlobalFiler™ kit again elevated its CPI ≥ 10,000 while the Identifiler Plus kit was only 30. Furthermore, GlobalFiler™ kit can expose the genotyping of 21 autosome, Amelogenin, Y-indel and DYS391, especially with the last two exclusive loci for the kit to be capable of determining gender when samples are even of Y-allele-absence in Amelogenin. Conclusions GlobalFiler™ kit, capable of amplifying more loci in a single run of amplification than the widely used Identifiler Plus kit, has greatly increased the CPI for identifying unknown bodies. Moreover, its additional Y-chromosome loci can help determine gender more effectively. Therefore, GlobalFiler™ kit is high applicable in identifying unknown bodies.关键词
法医遗传学/STR 基因座/Y 等位基因缺失/GlobalFiler™ 试剂盒/骨骼/牙齿/累积父权指数Key words
forensic genetics/STR loci/Y-allele absence/GlobalFiler™ kit/bone/teeth/cumulative paternity index (CPI)分类
社会科学引用本文复制引用
任文彦,郝宏蕾,王怀锋,吴微微,苏艳佳..GlobalFiler™试剂盒在尸骨鉴定中的应用[J].刑事技术,2015,(5):379-381,3.基金项目
公安部重点研究计划项目 （）
