新医学Issue(9)：580-583,4.DOI:10.3969/g.issn.0253-9802.2015.09.006
不明原因复发性自然流产患者 FOXP3基因蛋白表达与启动子甲基化水平之间关系的研究
Association between FOXP3 promoter methylation and the expression of FOXP3 protein in unexplained ;recurrent spontaneous abortion
摘要
Abstract
Objective To investigate the association between the level of FOXP3 promoter methyla-tion and the expression level of FOXP3 protein in the decidua of patients with unexplained recurrent spontane-ous abortion (URSA).Methods The expression levels of FOXP3 protein in the decidua of 20 URSA patients and 20 healthy counterparts who underwent induced abortion were assessed by Western blot.Bisulfite-assisted genomic sequencing PCR (BSP)was utilized to detect the levels of FOXP3 promoter methylation.The correla-tion between two indexes was evaluated.Results The expression level of FOXP3 in the decidua of the URSA group was significantly lower than that of the control group (P <0.01),whereas the level of FOXP3 promoter methylation in the URSA group was significantly higher compared with that in the control group (P <0.01).The expression level of FOXP3 protein was negatively correlated with the level of FOXP3 promoter methylation (r =-0.917,P <0.01).Conclusion As the level of FOXP3 promoter methylation increases,the expres-sion level of FOXP3 protein is down-regulated,which may cause abnormal immune tolerance and potentially lead to the incidence of RSA.关键词
不明原因复发性自然流产/叉头样转录因子 P3/基因启动子/甲基化Key words
Unexplained recurrent spontaneous abortion/FOXP3/Promoter/Methylation引用本文复制引用
侯文汇,李银广,李珠玉,李婕,方利元,李小青,游泽山..不明原因复发性自然流产患者 FOXP3基因蛋白表达与启动子甲基化水平之间关系的研究[J].新医学,2015,(9):580-583,4.基金项目
广东省自然科学基金 （）
