烟草科技2015，Vol.48Issue(11)：7-10,32,5.DOI:10.16135/j.issn1002-0861.20151102
无致病力青枯菌株对烟草青枯病的控制作用
Control Effects of Avirulent Strain Tbw1-7-3 of Ralstonia Solanacearum on Tobacco Bacterial Wilt
摘要
Abstract
To further reveal the control mechanism of avirulent strains of Ralstonia solanacearum against tobacco bacterial wilt, biocontrol trials were conducted in a greenhouse and field plots. The colonization of drug-resistant mutant strains in tobacco plants was determined via drug resistance marker, and the changes of activities of enzymes PAL, POD, PPO in the treated tobacco plants were analyzed. The results showed that the relative control efficacy of avirulent strain Tbw1-7-3 against tobacco bacterial wilt was up to 89.4% in greenhouse. At the early stage(10-20 days)after disease occurrence, the control efficacy of Tbw1-7-3 was comparable to that of streptomycin in fields, its drug-resistant mutant strain Tbw1-7-3k could colonize on the surface of tobacco roots and in tobacco plants for a short duration. Meanwhile, Tbw1-7-3 promoted the activities of PAL, POD, PPO and the accumulation of pathogenesis-related protein (PRP) in hosts. Avirulent strains possessed better control efficacy against tobacco bacterial wilt, especially strain Tbw1-7-3. The field experiments also indicated that Tbw1-7-3 had good control efficacy at the early stage of disease occurrence. The antagonistic mechanism of avirulent strains against Ralstonia solanacearum might be the results of both position occupying and resistance inducing.关键词
烟草青枯病/无致病力菌株/生物防治/控病机理Key words
Tobacco bacterial wilt/Avirulent strain/Biological control/Antagonistic mechanism分类
农业科技引用本文复制引用
陈国康,周帮菊,周丹妮,肖崇刚,马冠华,董国菊..无致病力青枯菌株对烟草青枯病的控制作用[J].烟草科技,2015,48(11):7-10,32,5.基金项目
重庆市烟草专卖局"重庆市烟草有害生物调查研究"(NY2010060103007) （NY2010060103007）
西南大学学科团队建设项目"作物病虫害成灾机理及持续控制的基础研究"(2362015XK04). （2362015XK04）
