烟草科技2015，Vol.48Issue(11)：11-15,5.DOI:10.16135/j.issn1002-0861.20151103
m型硫氧还蛋白对马铃薯Y病毒侵染烟草的影响
Effects of m-Type Thioredoxin on Potato Virus Y(PVY)Infected Tobacco
摘要
Abstract
In order to further confirm the interactive relationships between virus and its host, a gene featured the characters of m-type thioredoxin in tobacco cell was cloned by homologous alignment and named as NtTrm. The expression of NtTrm and its relationship with PVY accumulation were studied by protoplast transient transformation, virus-induced gene silencing (VIGS), semi-quantitative RT-PCR and Northern blot test technologies. The results showed that the expression of NtTrm in leaves of cv. Honghuadajinyuan was obviously up-regulated 10 days after PVY inoculation; the down-regulation of NtTrm significantly promoted the accumulation of PVY in leaves of Nicotiana benthamiana; while the over-expression of NtTrm in tobacco protoplast obviously inhibited the accumulation of PVY viral RNA. It preliminarily indicated that NTtrm could be induced by PVY infection and its up-regulated expression might affect the PVY infection in tobacco cells.关键词
烟草/m型硫氧还蛋白/马铃薯Y病毒/侵染Key words
Tobacco/m-Type thioredoxin/Potato virus Y(PVY)/Infection分类
农业科技引用本文复制引用
贾蒙骜,张荣春,商胜华,陈兴江,郭玉双,王仁刚..m型硫氧还蛋白对马铃薯Y病毒侵染烟草的影响[J].烟草科技,2015,48(11):11-15,5.基金项目
贵州省科学技术基金资助项目"贵州烟草马铃薯Y病毒分子变异和重组研究"(黔科合J字[2013]2194) （黔科合J字[2013]2194）
中国烟草总公司贵州省公司资助项目"贵州烟草有害生物调查研究"(2010-22)和"烟草抗性快速鉴定体系在抗病育种中的应用研究"(2009-11). （2010-22）
