临床医学工程Issue(10)：1278-1280,3.DOI:10.3969/j.issn.1674-4659.2015.10.1278
神经生长因子及其受体在EAE大鼠模型中的动态表达
Dynamic Expression of Nerve Growth Factor and Its Receptors in EAE Rats Model
摘要
Abstract
Objective To investigate the dynamic expression of nerve growth factor (NGF) and its receptor (TrkA and P75NTR) in experimental allergic encephalomyelitis (EAE). Methods 36 Lewis rats were given induced emulsion in subcutaneous injection by double foot pad and bordetella pertussis in intraperitoneal injection, to establish the EAE model. And the rats were randomly divided into initial phase group, peak phase group and paracmasis group, with 12 rats in each group; the other 12 Lewis rats were set as normal group. HE dyeing and immunohistochemistry were used to observe the pathological change and the expression of NGF, TrkA and P75NTR in brain and spinal tissue, TUNEL technique was used to detect the apoptosis of neuron. Results The expression of NGF and P75NTR in brain and spinal tissue increased with the development of clinical symptoms in EAE models, but the expression of TrkA decreased. The apoptosis of neuron was obviously related with the course of disease in EAE models, and statistical difference was found in different groups (P <0.05). Conclusions The expression of NGF, TrkA and P75NTR exists in EAE models, which plays an important role in the course of onset.关键词
实验变态反应性脑脊髓炎 (EAE)/神经生长因子/TrkA/P75NTR/凋亡Key words
Experimental allergic encephalomyelitis/Nerve growth factor/TrkA/P75NTR/Apoptosis分类
医药卫生引用本文复制引用
冯欣,高聪,谢富华,杨洁,区腾飞,林美容,占婷婷..神经生长因子及其受体在EAE大鼠模型中的动态表达[J].临床医学工程,2015,(10):1278-1280,3.基金项目
广东省自然科学基金项目(项目编号8151018201000036) （项目编号8151018201000036）
