云南民族大学学报（自然科学版）Issue(6)：442-448,7.DOI:12.3969/j.issn.1672-8513.2015.06.002
灯盏花 matK 基因序列分析及分子鉴定
Gene sequence analysis and molecular authentication of the matK gene of Erigeron breviscapus
摘要
Abstract
In its application,the medicinal plant Erigeron breviscapus will easily get mixed with plants of the Eeri-geron genus or Asters.This research focuses on the bioinformatic analysis of the matK gene of Erigeron breviscapus and its phytomorphology.The matK gene of Erigeron breviscapus was amplified and cloned by the PCR method be-fore its bioinformatic analysis.The results showed that the full length sequence of the matK gene of Erigeron brevis-capus was 1,317bp,and 438 encoded amino acids,with no signal peptide or transmembrane protein,and some hy-drophilic property.Its secondary structure is primarily an alpha helix and beta fold,and the subcellular localization confirmed the existence of the matK gene in the chloroplastid of eukaryotes with eight protein-binding sites and three polynucleotides.The combination of the methods of phytomorphology,the sequence variation of amino acids and the phylogenetic tree can distinguish Erigeron breviscapus from other plants.关键词
灯盏花matK基因/理化性质/系统进化树/分子鉴定Key words
matK gene of erigeron breviscapus/physicochemical property/phylogenetic tree/molecular authenti-cation分类
医药卫生引用本文复制引用
熊勇,杨威,雷懿,朱漫金,张雪娇,徐丽娟..灯盏花 matK 基因序列分析及分子鉴定[J].云南民族大学学报（自然科学版）,2015,(6):442-448,7.基金项目
国家自然科学基金（21462052）；云南民族大学学生实验实践能力提升工程项目；云南民族大学生物技术校级实践教学示范基地． （）
