亚热带植物科学Issue(1)：1-4,4.DOI:10.3969/j.issn.1009-7791.2013.01.001
‘解放钟’枇杷香树脂醇合成酶基因ejAS保守区的克隆
Clone of Amyrin Synthase Gene Conservative District from Eriobotrya japonica‘Jie Fang Zhong’
摘要
Abstract
Taking the leaves of loquat as materials, the improved method SDS was used for the RNA isolation. Based on the extracted total RNA, the AS gene conservative district sequence was cloned by the method of homology cloning and nested PCR. The results demonstrated that the conserved sequence was 854 bp in length, coded 284 amino acids and was named ejAS (NCBI number:JX173279.1). Though sequence analysis by Blast in NCBI, the results showed that the ejAS gene had the highest homology and similarity (98%) to the Malus×domestica, and shared above 70%homology with the reported Aralia elata, Glycyrrhiza uralensis, Polygala tenuifolia, etc.关键词
枇杷/AS基因/基因克隆/同源性Key words
loquat/AS gene/gene clone/homology分类
农业科技引用本文复制引用
刘小英,苏明华,吴少华,李惠华..‘解放钟’枇杷香树脂醇合成酶基因ejAS保守区的克隆[J].亚热带植物科学,2013,(1):1-4,4.基金项目
福建省自然科学基金(2012J05047)、福建省公益类科研院所专项(2011R1012-1) （2012J05047）
