医学信息Issue(14)：266-267,2.
梅毒螺旋体重组蛋白Tp1038的表达与鉴定
Expression and Identification of Recombinant Protein Tp1038 of Treponema Pallidum
摘要
Abstract
Objective To express and identify recombinant protein Tp1038 (rTp1038) of Treponema pal idum (Tp), providing a basis for further investigation of its significance in syphilis serodiagnosis. Methods PCR was used to amplify ful length of Tp1038 gene. The prokaryotic expression recombinant pET-28a (+)/Tp1038 was constructed and transformed into E.coli BL21 for expressing rTp1038. rTp1038 were purified with Ni-NTA af inity chromatography. SDS-PAGE was used to analyse expression form and purity of rTp1038. Western blot was performed to identify antigenicity of rTp1038. Results The prokaryotic recombinant pET-28a (+)/Tp1038 was constructed successful y and an approximate 20 KDa recombinant protein was expressed ef iciently in E.coli with dominant soluble form and more than 98% purity. Western blot indicated that purified proteins were able to be recognized special y by ant-His monoclonal antibody and pooled sera from syphilis patients. Conclusions The soluble recombinant Tp1038 are expressed ef iciently in E.coli and have good antigenicity and specifity.关键词
梅毒螺旋体/Tp1038/重组抗原/抗原性Key words
Treponema pal idum/Tp1038/Recombinant antigen/Antigenicity引用本文复制引用
刘小军,蒋传好,曾铁兵,赵飞骏,余坚,吴移谋..梅毒螺旋体重组蛋白Tp1038的表达与鉴定[J].医学信息,2013,(14):266-267,2.基金项目
国家自然科学基金(81273322)；湖南省自然科学基金重点项目(11JJ2044)；湖南省研究生培养创新项目 （81273322）
