摘要
Abstract
Objective To investigate the ef ect of prostaglandin E2 (PGE2) on the receptor of stromal cel -derived factor-1 (chemokine receptor 4, CXCR4)in Endometrial Cancer Ishikawa cel s. Methods Ishikawa cel s were treated with PGE2, EP1 receptor agonist, EP1 receptor antagonist, protein kinase C (PKC)inhibitor and Ca2+chelating agent, Real-time PCR,Western Blot were employed to detect the level of CXCR4 mRNA and CXCR4 protein in Ishikawa cel s. Results The mRNA level of CXCR4 increased 75.7% ( <0.05),while the level of CXCR4 protein increased 70.93% ( <0.01) after treated with 5μmol/L PGE2. When treated with 5μmol/L EP1 receptor agonist 17-PT-PGE2, the level of CXCR4 protein increased 84.82% ( <0.01).The protein level decreased%54.62%( <0.05) after treated with 10μmol/L EP1 receptor antagonist sc-51322 compared with the cel s treated with PGE2.When treated with 5μmol/L PKC inhibitor BIS-1, 10μmol/L Ca2+chelating agent BAPTA-AM, the protein levels of CXCR4 were decreased 50.17%( <0.05),56.33%( <0.05)compared with the cel s treated with 17-PT-PGE2. Conclusion PGE2 might up-regulate the expression level of CXCR4 through EP1 receptor which could be partly related to the Ca2+/PKC signaling pathway in Endometrial Cancer Ishikawa cel s.关键词
子宫内膜癌/PGE2/EP1受体/CXCR4/PKC/Ca2+Key words
Endometrial cancer/PGE2/EP1 receptor/CXCR4/PKC/Ca2+
