摘要
Abstract
Objective To investigate clinical value dif erences of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease including col oidal gold immunochromatography assay (GICA) and enzyme linked immunosorbent assay (ELISA). Methods 180 cases of children with early stage of foot and mouth disease were chosen in recent year in our hospital and detected anti EV-71-IGM antibody by col oidal gold immunochromatography assay (GICA) and enzyme linked immunosorbent assay (ELISA); and the positive detection rate and clinical diagnosis consistency of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease was compared. Results The detected coincidence rate of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease including GICA and ELISA was 76.67%(138/180)and Kappa value of clinical diagnosis consistency was 0.52. The positive detection rate of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease including GICA and ELISA were separately 47.78%(86/180), 26.67%(48/180);the positive detection rate of two kinds of detected methods for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease by GICA was significantly higher than ELISA( <0.05). Conclusion Compared with ELISA method, GICA method for detection on anti EV-71-IGM antibody of children with early stage of foot and mouth disease possess higher positive detection rate; and for children with negative detection results by two kinds of methods, it should be promptly review in order to avoid missing detection.关键词
GICA/ELISA/手足口病/抗EV-71-IGM抗体Key words
GICA/ELISA/Foot and mouth disease/Anti EV-71-IGM antibody
