医学信息Issue(43)：148-149,2.
缺氧复氧后内皮细胞中PBEF与VEGF、p-MLC的相关性研究
Study on the Relationship between p-MLC and VEGF and PBEF in Endothelial Cells after Hypoxia
摘要
Abstract
Objective To discuss the expression and relationship of pre-βcel colony-enhancing factor(PBEF),vessel endothelial growth factor(VEGF)and phosphorylation of myosin light chains(pMLC)in anoxia-reoxygenation of human umbilical vein endothelial cel (HUVEC).Methods HUVEC cel s were cultivated by the conventional method in vitro and divided into control,anoxia and anoxia-reoxygenation groups with 10 hole cel culture plate each.Expression of PBEF,VEGF and pMLC protein in every group were detected by western blot ing and analyzed the cor elation in anoxia-reoxygenation of HUVEC.Results Expression of PBEF,VEGF and pMLC protein were obviously higher than control group after HUVEC cel s of oxygen ( <0.01).Expression of PBEF,VEGF and pMLC protein increased more in HUVEC cel s of anoxia group after reoxygenation 12 h than anoxia group with statistical y significances ( <0.01).There are positive cor elations among expression of PBEF, VEGF and pMLC protein in HUVEC anoxia-reoxygenation.Rank cor elation coef icient was 0.493 between PBEF and VEG.Rank cor elation coef icient was 0.534 between PBEF and pMLC.Rank cor elation coef icient was 0.469 between VEGF and pMLC.Conclusion PBEF,VEGF and pMLC participated HUVEC anoxia-reoxygenation process and had a significant cor elation in HUVEC anoxia-reoxygenation.关键词
前β细胞克隆增强因子/血管内皮生长因子/肌球蛋白轻链磷酸化/缺氧复氧Key words
Pre-βcel colony-enhancing factor/Vessel endothelial growth factor/Phosphorylation of myosin light chains/Anoxia-reoxygenation引用本文复制引用
杨威,董啸..缺氧复氧后内皮细胞中PBEF与VEGF、p-MLC的相关性研究[J].医学信息,2015,(43):148-149,2.基金项目
江西省教育厅基金项目编号00016684资助 （）
