医学信息Issue(45)：36-36,1.
宫颈鳞癌SiHa细胞中HIF-1a基因、survivin基因的表达
Expression of HIF-1a Gene and Survivin Gene in Squamous Cell Carcinoma of Cervix
摘要
Abstract
Objective Discussion SiHa cervical squamous carcinoma cel s HIF -1α gene (HIF-1α) relationship with survivin's anti-apoptotic gene survivin gene. Methods This study through with cobalt chloride simulated hypoxia to promote the expression of HIF-1a in cultured cervical squamous cel carcinoma cel line SiHa, again by RNA interference inhibits the expression of HIF-1a gene, by Western blot method was used to detect the HIF-1a gene and survivin gene in the lack of oxygen condition expression. Results After incubation under hypoxic state and increased expression of HIF-1αin cervical squamous cel carcinoma SiHa cel s, survivin protein expression was also increased, and with the lack of oxygen concentration dependent;RNA interference to reduce the expression method of inhibiting HIF-1α gene in cel s HIF-1α , survivin protein expression is reduced. Conclusion In squamous cel carcinoma SiHa cel s, the gene expression of HIF-1α and survivin highly relevant, inhibition of HIF-1αexpression of survivin by inhibition of apoptosis may promote tumor cel s.关键词
缺氧诱导因子1-α亚基/宫颈肿瘤/生存素/乏氧/细胞凋亡中图分类号院R 737.33Key words
HIF-1α/Cervical cancer/Survivin/Hypoxia/Apoptosis引用本文复制引用
古力米热·布然江,艾力克木·阿不都玩克,李小文..宫颈鳞癌SiHa细胞中HIF-1a基因、survivin基因的表达[J].医学信息,2015,(45):36-36,1.基金项目
新疆维吾尔自治区自然科学基金面上项目编号2012211A1047。 （）
