医药导报Issue(10)：1272-1275,4.DOI:10.3870/j.issn.1004-0781.2015.10.002
熄风通脑胶囊对局灶性脑缺血-再灌注损伤大鼠脑组织TNF-α和IL-1β的影响∗
Effects of Xifeng Tongnao Capsules on Expression of TNF-αand IL-1βin Brain Tissue of Rats with Focal Cerebral Ischemia-Reperfusion Injury
摘要
Abstract
Objective To observe the effects of Xifeng Tongnao capsules on the content of TNF-α and IL-1β in brain tissue of rats with focal cerebral ischemia-reperfusion injury. Methods A total of 120 adult SD rats were randomized into 6 groups: Xifeng Tongnao low-, middle- and high-dose groups, model control group, Buchang Naoxintong group and sham-operated group.Buchang Naoxintong group were treated with Buchang Naoxintong capsules at 0.864 g��kg-1.Xifeng Tongnao high-, middle-and low-dose groups were treated with 3.456, 1.728, and 0.864 g��kg-1 Xifeng Tongnao capsules, respectively;sham-operated group and model control group were treated with equal volume of purified water. Medications were administered intragastrically once daily for 7 days. The acute transient focal cerebral ischemia-reperfusion model was established by middle cerebral artery occlusion ( MCAO) 1 h after the final dose, and rats in the sham-operated group only received anesthesia and stripping without occlusion.All rats were sacrificed after reperfusion for 24 h, and expression levels of TNF-α and IL-1β in the brain tissue were detected by ELISA. Results TNF-αcontent in Xifeng Tongnao capsutes low-, middle-and high-dose groups were (35.34±8.95), (33.75±6.92), and (40.95±5.39) ng��L-1, respectively.IL-1β content were (1.44±0.47), (1.45± 0.23), and (1.61±0.33) ng��L-1 in low-, middle- and high-dose groups, respectively.TNF-α and IL-1β were (38.96±9.84) and (1.56±0.31) ng��L-1, respectively in Buchang Naoxintong group, (52.74±6.76) and (2.79±0.45) ng��L-1in the model control group, and (32.54±4.00) and (1.32±0.22) ng��L-1 in sham-operated group.TNF-αand IL-1βcontent were significantly higher in Buchang Naoxintong group than in sham-operated group ( P<0. 05 ) . TNF-α and IL-1β content were significantly decreased in Xifeng Tongnao high-, middle- and low-dose groups (all P<0.05). Conclusion Xifeng Tongnao capsules can protect brain tissue by supressing TNF-α and IL-1β and alleviating inflammatory injury from ischemia.关键词
熄风通脑胶囊/损伤,脑缺血-再灌注/肿瘤坏死因子/白细胞介素Key words
Xifeng Tongnao capsules/Injury, cerebral ischemia-reperfusion/Tumor necrosis factor/Interleukin分类
医药卫生引用本文复制引用
曾聪彦,胡莹,高玉桥,梅全喜,林慧,戴卫波,张文霞..熄风通脑胶囊对局灶性脑缺血-再灌注损伤大鼠脑组织TNF-α和IL-1β的影响∗[J].医药导报,2015,(10):1272-1275,4.基金项目
广东省中山市科技计划资助项目(20122A010) （20122A010）
