医药导报Issue(10)：1347-1351,5.DOI:10.3870/j.issn.1004-0781.2015.10.023
桔梗总皂苷提取工艺优化∗
Optimized Extraction of Total Saponins from Platycodon grandiflorum
摘要
Abstract
Objective To optimize the process of extracting total saponins from Platycodon grandiflorum by orthogonal experimental design and central composite design-response surface method. Methods Independent variables were concentration of ethanol, reflux extraction time, reflux time and solvent fold. Dependent variables were extraction rate of total saponins in Platycodon grandiflorum and the yield of dry extract.Linear or nonlinear mathematic models were used to estimate the relationship between independent and dependent variables. Results The result of the orthogonal experimental design was 70%ethanol, 180 min for reflux, 6 fold of solvent and 2 times for extraction. The result of central composite design-response surface method was 70% ethanol, 140 min for reflux, 10 fold of solvent and 2 times for extraction. Conclusion The central composite design-response surface method is better than orthogonal experimental design in studying extraction process of Platycodon grandiflorum, which provides basis for feasibility of application of central composite design-response surface method in optimization of extraction process of Chinese traditional medicine.关键词
桔梗/桔梗总皂苷/正交设计/星点设计-效应面法/提取工艺Key words
Platycodon grandiflorum/Total saponins of Platycodon grandiflorum/Orthogonal experimental design/Central composite design-response surface method/Extraction process分类
医药卫生引用本文复制引用
李喜凤,安硕,张伟晓,王优湑,贾永艳..桔梗总皂苷提取工艺优化∗[J].医药导报,2015,(10):1347-1351,5.基金项目
河南省科技厅重点科技攻关项目(14104151)；河南省教育厅科技攻关项目(14202117) （14104151）
