医药导报Issue(10)：1352-1355,4.DOI:10.3870/j.issn.1004-0781.2015.10.024
正交实验法优化姜黄中姜黄素提取工艺及其抗氧化活性∗
Optimized Extraction of Curcuminoids from Curcuma Longa L.by Orthogonal Designed Method and Its Antioxidant Activity
摘要
Abstract
Objective To investigate the optimal extraction technique and antioxidant activity of curcuminoids from Curcuma longa L. Methods The optimized extraction process of curcuminoids from Curcuma longa L. with ethanol as extracting solvent were studied based on orthogonal test.The effect of each factor such as the ethanol concentration, the amount of solvent, extraction temperature and extraction time were investigated based on a single-factor experiment.The effects of extraction yield of curcuminoids under different processing conditions were compared by HPLC method. Content of curcuminoids and antioxidant activity of the extract were used as comprehensive survey index. Free radical scavenging DPPH method was used to assess the antioxidant effect. And the extraction technique parameters were optimized. Results The condition of optimal extraction technique was: ethanol concentration 60%, amount of solvent 20 folds, extraction time 2 h, extraction temperature 90 ℃. Under these conditions, the extraction yield of curcuminoids was 3. 65%. DPPH free radical scavenging yield of the curcumin extract was 79.79%. Conclusion This optimized technology is simple and have a good reappearing rate, and the extracts of Curcuma longa L.show good antioxidant activity.关键词
姜黄素/DPPH法/提取工艺/抗氧化活性Key words
Curcuminoids/DPPH radical assay/Orthogonal array design/Antioxidant activity分类
医药卫生引用本文复制引用
周美,陈华国,周欣,龚小见..正交实验法优化姜黄中姜黄素提取工艺及其抗氧化活性∗[J].医药导报,2015,(10):1352-1355,4.基金项目
贵阳市科技计划项目(筑科合同[2012203]2-15)；贵州省教育厅特色重点实验室项目(黔科合 KY[2012]005)；贵州省药物质量控制及评价技术工程实验室项目(黔发改高技[2013]2068号)；贵州省中药材现代产业技术体系建设专项( GZCYTX-02)。 （筑科合同[2012203]2-15）
