医药导报Issue(11)：1483-1485,3.DOI:10.3870/j.issn.1004-0781.2015.11.018
壮药战骨中柚皮素与芹菜素含测定量∗
Determination of Naringenin and Apigenin in Premna fulva Craib.by HPLC
摘要
Abstract
Objective To establish a HPLC method for determination of naringenin and apigenin in Premna fulva. Methods The SHISEIDO￣SPOLAR C18(250 mm×4.6 mm,5μm) was used as analytical column.The mobile phase consisted of methanol￣0.2% phosphoric acid (42:58) with isocratic elution at a flow rate of 1.0 mL.min-1 .The detection wavelength of naringenin and apigenin was 288 nm and 340 nm, respectively.Column temperature was set at 35 ℃ , the injection volumn was 10 μL. Results Naringenin and apigenin had a good linear relationship in the concentration range of 0.180 ~ 3.60 μg (r =0.999 9) and 0. 0052 ~ 0. 1040 μg ( r = 0. 999 9), respectively. Conclusion The method is accurate and reliable. It is appropriate for the quantitative determination of naringenin and apigenin in Premna fulva and its preparations.关键词
战骨/柚皮素/芹菜素/色谱法,高效液相/含量测定Key words
Premna fulva Craib./Naringenin/Apigenin/Chromatography, high performance liquid/Content determination分类
医药卫生引用本文复制引用
叶勇,黄秋洁,刘华钢,陈光强,农玉红,杨丽婷,钟锦华..壮药战骨中柚皮素与芹菜素含测定量∗[J].医药导报,2015,(11):1483-1485,3.基金项目
广西自然科学基金资助项目(2013GXNSFBA019149)；广西高校科技创业能力提升工程专项项目(70￣ZJGX201402003)；广西医科大学大学生创新创业训练计划项目(2013CXCY118)；广西医科大学未来学术之星大学生课外科研课题(WLXSZX1403) （2013GXNSFBA019149）
