中国病理生理杂志Issue(11)：2016-2020,5.DOI:10.3969/j.issn.1000-4718.2015.11.015
高糖激活Ca2＋－CaN－NFAT3信号通路致H9c2细胞肥大
High glucose induces H9 c2 cardiomyocyte hypertrophy through Ca2+-CaN-NFAT3 signaling pathway
徐小红 1阮骆阳 1田小华 1潘凤娟 1杨彩兰 2柳国胜2
作者信息
- 1. 广东省农垦中心医院儿科，广东湛江524002
- 2. 暨南大学第一临床医学院儿科，广东广州510632
- 折叠
摘要
Abstract
AIM:To study the morphological changes of cardiac H9c2 cells during the developmental process of fetal rat.METHODS:Embryonic rat heart-derived H9c2 cells were maintained in DMEM supplemented with 10%fetal bovine serum.The H9c2 cells were plated at a density of 6 000 cells/cm and divided into 5 groups:H9c2 cells were trea-ted with 5 mmol/L glucose, 25 mmol/L glucose, 50 mmol/L glucose, Norvasc (25 nmol/L) +25 mmol/L glucose, or Norvasc (25 nmol/L)+50 mmol/L glucose for 48 h.The morphology of H9c2 cells was observed.The cell surface area was measured by Image-Pro Plus 6.1 software.Fluorescence spectrophotometry was used to detect the concentration of in-tracellular calcium ion ( [ Ca2+] i ) in the cardiomyocytes.The concentration of CaN in the cell was measured by ELISA. The mRNA expression of CaNAβ, NFAT3 and β-MHC in the cells was detected by real-time PCR.The protein levels of CaNAβ, NFAT3 and β-MHC in cultural H9c2 cells were detected by Western blot.RESULTS: The mean area of the cells, the mean fluorescence value of [ Ca2+] i and the concentration of CaN in 25 mmol/L glucose group were higher than those in 5 mmol/L glucose group, and those were lower than those in 50 mmol/L glucose group.After treated with Nor-vasc, those results decreased significantly.The expression of CaNAβ, NFAT3 andβ-MHC at mRNA and protein levels in 25 mmol/L glucose group was higher than those in 5 mmol/L glucose group, but was lower than those in 50 mmol/L glu-cose group .The expression of CaNAβ, NFAT3 andβ-MHC at mRNA and protein levels decreased significantly in Norvasc treatment group.CONCLUSION:Ca2+-CaN-NFAT3 signaling pathway is perhaps involved in high glucose-induced H9c2 cardiomyocyte hypertrophy.关键词
妊娠期糖尿病/心肌细胞肥大/氨氯地平/钙调神经磷酸酶/活化T细胞核因子3Key words
Gestational diabetes mellitus/Cardiomyocyte hypertrophy/Amlodipine/Calcineurin/Nuclear factor of activated T cells 3分类
医药卫生引用本文复制引用
徐小红,阮骆阳,田小华,潘凤娟,杨彩兰,柳国胜..高糖激活Ca2＋－CaN－NFAT3信号通路致H9c2细胞肥大[J].中国病理生理杂志,2015,(11):2016-2020,5.
