中国病理生理杂志Issue(11)：2047-2052,6.DOI:10.3969/j.issn.1000-4718.2015.11.020
m iR－21在周围神经损伤时调控雪旺细胞凋亡
miR-21 inhibits apoptosis of Schwann cells following peripheral nerve in-jury
摘要
Abstract
AIM:To explore the relationship and molecular mechanism between microRNA-21(miR-21) and Schwann cells ( SC) following peripheral nerve injury.METHODS: The mRNA expression of miR-21 and phosphatase and tensin homologue deleted on chromosome ten ( PTEN) in animal model were detected by real-time PCR.The over-ex-pression of miR-21 and inhibition of miR-21 expression in the Schwann cells according to transfection of lentiviral vectors were performed, the nonspecific miRNA was used as a negative control ( NC) .The cell apoptosis was measured by flow cy-tometry.The mRNA expression of miR-21 and PTEN in the cells was detected by real-time PCR.The protein expression of PTEN and cleaved caspase-3 was determined by Western blot.RESULTS: The level of miR-21 was significantly higher and the mRNA level of PTEN was significantly lower in the model of nerve injury than those in control group.miR-21 over-expression decreased the number of apoptotic Schwann cells compared with NC-SC.The mRNA expression of PTEN was down-regulated by over-expression of miR-21.The protein expression of PTEN and cleaved caspase-3 was down-regulated by over-expression of miR-21 (P<0.05).CONCLUSION: miR-21 may play an important role in the peripheral nerve injury through inhibiting apoptosis of Schwann cells by down-regulating the expression of PTEN.关键词
周围神经损伤/微小RNA-21/雪旺细胞/细胞凋亡Key words
Peripheral nerve injury/MicroRNA-21/Schwann cells/Apoptosis分类
医药卫生引用本文复制引用
陆新华,王辉,宁昕杰,罗骏成,梁家骥..m iR－21在周围神经损伤时调控雪旺细胞凋亡[J].中国病理生理杂志,2015,(11):2047-2052,6.基金项目
国家自然科学基金资助项目（No．30901542）；广东省自然科学基金自由申请项目（No．2014A030313055）；产学研合作项目（No．2014B090901043）；广东省科技计划（No．2012B031800056）；中山大学高校基本业务费青年教师培育资助项目 （）
