中国病理生理杂志Issue(11)：2053-2058,6.DOI:10.3969/j.issn.1000-4718.2015.11.021
蛇床子素对转染APP595／596基因的SH－SY5Y细胞的保护作用
Protective effect of osthole on SH-SY5Y cells transfected with APP595/596 gene
摘要
Abstract
AIM:To explore the protective effect of osthole on the SH-SY5Y cells transfected with APP595/596 gene, and to investigate the molecular mechanism.METHODS:The SH-SY5Y cells were transfected with APP595/596 gene in vitro for establishing a cell model to study the pathogenic role of amyloid β-protein ( Aβ) .The cell viability was detected by CCK-8 assay.The release of lactate dehydrogenase ( LDH) was determined by the colour reaction of dia-phorase-INT.The cell apoptotic rate was analyzed by flow cytometry.The expression of β-site APP cleaving enzyme 1 ( BACE1) at mRNA and protein levels was detected by RT-PCR and Western blot.The expression of Aβwas measured by the technique of immunofluorescence cytochemistry and Western blot.RESULTS: Treatment with osthole inhibited the LDH release, and increased the viability of the cells.The percentage of apoptotic cells was also significantly decreased. Osthole also inhibited the expression of BACE1 at mRNA and protein levels and the protein expression of Aβ.CONCLU-SION:Osthole has protective effect on SH-SY5Y cells transfected with APP595/596 gene.The mechanism may be associ-ation with inhibiting the mRNA and protein expression of BACE1.关键词
蛇床子素/APP595/596基因/β位点APP裂解酶1Key words
Osthole/APP595/596 gene/β-site APP cleaving enzyme 1分类
医药卫生引用本文复制引用
教亚男,姚璎珈,孔亮,李少恒,陶震宇,闫宇辉,杨静娴..蛇床子素对转染APP595／596基因的SH－SY5Y细胞的保护作用[J].中国病理生理杂志,2015,(11):2053-2058,6.基金项目
国家自然科学基金资助项目 （）
